Inhibition of beta-catenin signaling by Pb leads to incomplete fracture healing.

Eric E. Beier, Tzong Jen Sheu, Taylor Buckley, Kiminori Yukata, Regis O'Keefe, Michael J. Zuscik, J. Edward Puzas

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

There is strong evidence in the clinical literature to suggest that elevated lead (Pb) exposure impairs fracture healing. Since Pb has been demonstrated to inhibit bone formation, and Wnt signaling is an important anabolic pathway in chondrocyte maturation and endochondral ossification, we investigated the impact of Wnt therapy on Pb-exposed mice undergoing bone repair in a mouse tibial fracture model. We established that tibial fracture calluses from Pb-treated mice were smaller and contained less mineralized tissue than vehicle controls. This resulted in the persistence of immature cartilage in the callus and decreased β-catenin levels. Reduction of β-catenin protein was concurrent with systemic elevation of LRP5/6 antagonists DKK1 and sclerostin in Pb-exposed mice throughout fracture healing. β-catenin stimulation by the GSK3 inhibitor BIO reversed these molecular changes and restored the amount of mineralized callus. Overall, Pb is identified as a potent inhibitor of endochondral ossification in vivo with correlated effects on bone healing with noted deficits in β-catenin signaling, suggesting the Wnt/β-catenin as a pivotal pathway in the influence of Pb on fracture repair.

Original languageEnglish
Pages (from-to)1397-1405
Number of pages9
JournalJournal of orthopaedic research : official publication of the Orthopaedic Research Society
Volume32
Issue number11
DOIs
StatePublished - Nov 2014

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