ING2 as a novel mediator of transforming growth factor-β-dependent responses in epithelial cells

Krishna P. Sarker, Hiromi Kataoka, Angela Chan, Stuart J. Netherton, Isabelle Pot, Mai Anh Huynh, Xiaolan Feng, Azad Bonni, Karl Riabowol, Shirin Bonni

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

Members of the ING (inhibitor of growth) family of chromatin modifying proteins (ING1-ING5) have emerged as critical regulators of gene expression and cellular responses, suggesting that the ING proteins may impinge on specific signal transduction pathways and their mediated effects. Here, we demonstrate a role for the protein ING2 in mediating responses by the transforming growth factor (TGF)-β-Smad signaling pathway. We show that ING2 promotes TGF-β-induced transcription. Both gain-of-function and RNA interference-mediated knockdown of endogenous ING2 reveal that ING2 couples TGF-β signals to the induction of transcription and cell cycle arrest. We also find that the Smad-interacting transcriptional modulator SnoN interacts with ING2 and promotes the assembly of a protein complex containing SnoN, ING2, and Smad2. Knockdown of endogenous SnoN blocks the ability of ING2 to promote TGF-β-dependent transcription, and conversely expression of SnoN augments ING2 enhancement of the TGF-β response. Collectively, our data suggest that ING2 collaborates with SnoN to mediate TGF-β-induced Smad-dependent transcription and cellular responses.

Original languageEnglish
Pages (from-to)13269-13279
Number of pages11
JournalJournal of Biological Chemistry
Volume283
Issue number19
DOIs
StatePublished - May 9 2008

Fingerprint Dive into the research topics of 'ING2 as a novel mediator of transforming growth factor-β-dependent responses in epithelial cells'. Together they form a unique fingerprint.

  • Cite this

    Sarker, K. P., Kataoka, H., Chan, A., Netherton, S. J., Pot, I., Huynh, M. A., Feng, X., Bonni, A., Riabowol, K., & Bonni, S. (2008). ING2 as a novel mediator of transforming growth factor-β-dependent responses in epithelial cells. Journal of Biological Chemistry, 283(19), 13269-13279. https://doi.org/10.1074/jbc.M708834200