Influenza A virus specific T cell immunity in humans during aging

A. C.M. Boon; E. Fringuelli; Y. M.F. Graus; R. A.M. Fouchier; K. Sintnicolaas; A. M. Iorio; G. F. Rimmelzwaan; A. D.M.E. Osterhaus

doi:10.1006/viro.2002.1491

Influenza A virus specific T cell immunity in humans during aging

A. C.M. Boon, E. Fringuelli, Y. M.F. Graus, R. A.M. Fouchier, K. Sintnicolaas, A. M. Iorio, G. F. Rimmelzwaan, A. D.M.E. Osterhaus

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Abstract

To study the decreasing responsiveness of the immune system during aging, influenza virus specific cellular immunity was investigated in a cohort of healthy blood donors between 18 and 70 years of age. The percentage of influenza A virus specific T cells was determined by flow cytometry and found not to change during aging. After stimulation with phorbol 12-myristate 13-acetate and ionomycin, an increase in the percentage of IFN-γ and IL-4 producing CD8⁺ T cells was observed during aging. In addition, the cytotoxic T lymphocyte (CTL) activity was investigated in two additional groups of five donors, 18-20 and 68-70 years of age. The lytic capacity of purified CD8⁺ T cells, after in vitro stimulation of peripheral blood mononuclear cells with influenza A virus, seemed lower in 68- to 70-year-old donors than in 18- to 20-year-old donors. Therefore we conclude that the reduced CTL activity in the elderly is not the result of a lower frequency of virus-specific T cells, but more likely the result of impaired antigen-specific proliferation or lower lytic capacity of these cells.

Original language	English
Pages (from-to)	100-108
Number of pages	9
Journal	Virology
Volume	299
Issue number	1
DOIs	https://doi.org/10.1006/viro.2002.1491
State	Published - 2002

Keywords

Aging
Cytotoxic T-lymphocyte
Flow cytometry
Immunosenescence
Influenza virus

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10.1006/viro.2002.1491

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@article{cece225e6f374d08a5167c79e3970f7c,

title = "Influenza A virus specific T cell immunity in humans during aging",

abstract = "To study the decreasing responsiveness of the immune system during aging, influenza virus specific cellular immunity was investigated in a cohort of healthy blood donors between 18 and 70 years of age. The percentage of influenza A virus specific T cells was determined by flow cytometry and found not to change during aging. After stimulation with phorbol 12-myristate 13-acetate and ionomycin, an increase in the percentage of IFN-γ and IL-4 producing CD8+ T cells was observed during aging. In addition, the cytotoxic T lymphocyte (CTL) activity was investigated in two additional groups of five donors, 18-20 and 68-70 years of age. The lytic capacity of purified CD8+ T cells, after in vitro stimulation of peripheral blood mononuclear cells with influenza A virus, seemed lower in 68- to 70-year-old donors than in 18- to 20-year-old donors. Therefore we conclude that the reduced CTL activity in the elderly is not the result of a lower frequency of virus-specific T cells, but more likely the result of impaired antigen-specific proliferation or lower lytic capacity of these cells.",

keywords = "Aging, Cytotoxic T-lymphocyte, Flow cytometry, Immunosenescence, Influenza virus",

author = "Boon, {A. C.M.} and E. Fringuelli and Graus, {Y. M.F.} and Fouchier, {R. A.M.} and K. Sintnicolaas and Iorio, {A. M.} and Rimmelzwaan, {G. F.} and Osterhaus, {A. D.M.E.}",

note = "Funding Information: Part of this work was supported by the foundation for Respiratory Virus Infections, Notably Influenza (SRVI), and Numico Research BV. We also acknowledge Liane van de Kemp for growing and purifying the influenza A viruses and Marianne Baars for performing the influenza virus serology. Furthermore, we thank Saskia Sakko from the Blood-bank Rotterdam for supplying the buffy coats and blood sam ples and Dr. W. E. P. Beyer for statistical analysis. Dr. R. A. M. Fouchier is a fellow of the Royal Dutch Academy of Arts and Sciences",

year = "2002",

doi = "10.1006/viro.2002.1491",

language = "English",

volume = "299",

pages = "100--108",

journal = "Virology",

issn = "0042-6822",

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T1 - Influenza A virus specific T cell immunity in humans during aging

AU - Boon, A. C.M.

AU - Fringuelli, E.

AU - Graus, Y. M.F.

AU - Fouchier, R. A.M.

AU - Sintnicolaas, K.

AU - Iorio, A. M.

AU - Rimmelzwaan, G. F.

AU - Osterhaus, A. D.M.E.

N1 - Funding Information: Part of this work was supported by the foundation for Respiratory Virus Infections, Notably Influenza (SRVI), and Numico Research BV. We also acknowledge Liane van de Kemp for growing and purifying the influenza A viruses and Marianne Baars for performing the influenza virus serology. Furthermore, we thank Saskia Sakko from the Blood-bank Rotterdam for supplying the buffy coats and blood sam ples and Dr. W. E. P. Beyer for statistical analysis. Dr. R. A. M. Fouchier is a fellow of the Royal Dutch Academy of Arts and Sciences

PY - 2002

Y1 - 2002

N2 - To study the decreasing responsiveness of the immune system during aging, influenza virus specific cellular immunity was investigated in a cohort of healthy blood donors between 18 and 70 years of age. The percentage of influenza A virus specific T cells was determined by flow cytometry and found not to change during aging. After stimulation with phorbol 12-myristate 13-acetate and ionomycin, an increase in the percentage of IFN-γ and IL-4 producing CD8+ T cells was observed during aging. In addition, the cytotoxic T lymphocyte (CTL) activity was investigated in two additional groups of five donors, 18-20 and 68-70 years of age. The lytic capacity of purified CD8+ T cells, after in vitro stimulation of peripheral blood mononuclear cells with influenza A virus, seemed lower in 68- to 70-year-old donors than in 18- to 20-year-old donors. Therefore we conclude that the reduced CTL activity in the elderly is not the result of a lower frequency of virus-specific T cells, but more likely the result of impaired antigen-specific proliferation or lower lytic capacity of these cells.

AB - To study the decreasing responsiveness of the immune system during aging, influenza virus specific cellular immunity was investigated in a cohort of healthy blood donors between 18 and 70 years of age. The percentage of influenza A virus specific T cells was determined by flow cytometry and found not to change during aging. After stimulation with phorbol 12-myristate 13-acetate and ionomycin, an increase in the percentage of IFN-γ and IL-4 producing CD8+ T cells was observed during aging. In addition, the cytotoxic T lymphocyte (CTL) activity was investigated in two additional groups of five donors, 18-20 and 68-70 years of age. The lytic capacity of purified CD8+ T cells, after in vitro stimulation of peripheral blood mononuclear cells with influenza A virus, seemed lower in 68- to 70-year-old donors than in 18- to 20-year-old donors. Therefore we conclude that the reduced CTL activity in the elderly is not the result of a lower frequency of virus-specific T cells, but more likely the result of impaired antigen-specific proliferation or lower lytic capacity of these cells.

KW - Aging

KW - Cytotoxic T-lymphocyte

KW - Flow cytometry

KW - Immunosenescence

KW - Influenza virus

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DO - 10.1006/viro.2002.1491

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SN - 0042-6822

VL - 299

SP - 100

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JO - Virology

JF - Virology

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ER -

Influenza A virus specific T cell immunity in humans during aging

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Keywords

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