@article{f05270dbc30e476f92bd5ac29a4fe36e,
title = "Influence of fruit and invertebrate consumption on the gut microbiota of wild white-faced capuchins (Cebus capucinus)",
abstract = "Objectives: Invertebrate consumption is thought to be an integral part of early hominin diets, and many modern human populations regularly consume insects and other arthropods. This study examines the response of gut microbial community structure and function to changes in diet in wild white-faced capuchins (Cebus capucinus), a primate that incorporates a large proportion of invertebrates in its diet. The goal of the study is to better understand the role of both fruit and invertebrate prey consumption on shaping primate gut microbiomes. Materials and methods: Fecal samples (n = 169) and dietary data were collected over 12 months. The V3-V5 region of microbial 16S rRNA genes was amplified and sequenced. The IM-TORNADO pipeline was used to analyze sequences. Results: White-faced capuchin gut bacterial communities were characterized primarily by Firmicutes (41.6%) and Proteobacteria (39.2%). There was a significant relationship between the invertebrate diet composition of individual capuchins and their gut microbiome composition. However, there was no relationship between the fruit diet composition of individual capuchins and their gut microbiome composition, even when examining multiple timescales. Discussion: The results of our study indicate that there is a stronger relationship between gut microbial community structure and invertebrate diet composition than between gut microbial community structure and fruit consumption. As invertebrates and other animal prey play an important role in the diet of many primates, these results give important insight into the role of faunivory in shaping the evolution of host-microbe interactions in primates.",
keywords = "gut microbiome, human evolution, insectivory, omnivory, white-faced capuchins",
author = "Mallott, {Elizabeth K.} and Amato, {Katherine R.} and Garber, {Paul A.} and Malhi, {Ripan S.}",
note = "Funding Information: Thank you to Maderas Rainforest Conservancy and the Molina family for allowing us to conduct research at LSBFS. Cindy Taylor, Liz Rasheed, Michaela Snead, and Elizabeth Clausing were indispensable in the field and lab. PAG wishes to thank Chrissie, Sara, and Jenni. Thank you to the Roy J. Carver Biotechnology Center at UIUC, especially Chris Wright, Mark Band, and Chris Fields. This study was supported with funds from the UIUC Department of Anthropology, the Beckman Institute at UIUC, the Center for Latin American Studies at UIUC, the NSF GRFP program, and the Lewis and Clark Fund for Exploration and Field Research awarded to EKM, and funds awarded to PAG and RSM by the University of Illinois Urbana-Champaign. EKM conducted field research, laboratory work, the molecular analysis, and the statistical analyses. RSM contributed to the molecular protocols. KRA contributed to the statistical analyses. EKM, RSM, and PAG contributed to experimental design. EKM, RSM, PAG, and KRA contributed to the manuscript production. Sequence data is available in the Sequence Read Archive (http:// www.ncbi.nlm.nih.gov/sra), BioProject ID PRJNA299397. Funding Information: Thank you to Maderas Rainforest Conservancy and the Molina family for allowing us to conduct research at LSBFS. Cindy Taylor, Liz Rasheed, Michaela Snead, and Elizabeth Clausing were indispensable in the field and lab. PAG wishes to thank Chrissie, Sara, and Jenni. Thank you to the Roy J. Carver Biotechnology Center at UIUC, especially Chris Wright, Mark Band, and Chris Fields. This study was supported with funds from the UIUC Department of Anthropology, the Beckman Institute at UIUC, the Center for Latin American Studies at UIUC, the NSF GRFP program, and the Lewis and Clark Fund for Exploration and Field Research awarded to EKM, and funds awarded to PAG and RSM by the University of Illinois Urbana-Champaign. EKM conducted field research, laboratory work, the molecular analysis, and the statistical analyses. RSM contributed to the molecular protocols. KRA contributed to the statistical analyses. EKM, RSM, and PAG contributed to experimental design. EKM, RSM, PAG, and KRA contributed to the manuscript production. Sequence data is available in the Sequence Read Archive (http://www.ncbi.nlm.nih.gov/sra), BioProject ID PRJNA299397. Publisher Copyright: {\textcopyright} 2018 Wiley Periodicals, Inc.",
year = "2018",
month = mar,
doi = "10.1002/ajpa.23395",
language = "English",
volume = "165",
pages = "576--588",
journal = "American Journal of Physical Anthropology",
issn = "0002-9483",
number = "3",
}