Inferred metagenomic comparison of mucosal and fecal microbiota from individuals undergoing routine screening colonoscopy reveals similar differences observed during active inflammation

Mei San Tang, Jordan Poles, Jacqueline M. Leung, Martin J. Wolff, Michael Davenport, Soo Ching Lee, Yvonne Al Lim, Kek Heng Chua, P'ng Loke, Ilseung Cho

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

The mucosal microbiota lives in close proximity with the intestinal epithelium and may interact more directly with the host immune system than the luminal/fecal bacteria. The availability of nutrients in the mucus layer of the epithelium is also very different from the gut lumen environment. Inferred metagenomic analysis for microbial function of the mucosal microbiota is possible by PICRUSt. We recently found that by using this approach, actively inflamed tissue of ulcerative colitis (UC) patients have mucosal communities enriched for genes involved in lipid and amino acid metabolism, and reduced for carbohydrate and nucleotide metabolism. Here, we find that the same bacterial taxa (e.g. Acinetobacter) and predicted microbial pathways enriched in actively inflamed colitis tissue are also enriched in the mucosa of subjects undergoing routine screening colonoscopies, when compared with paired samples of luminal/fecal bacteria. These results suggest that the mucosa of healthy individuals may be a reservoir of aerotolerant microbial communities expanded during colitis.

Original languageEnglish
Pages (from-to)48-56
Number of pages9
JournalGut microbes
Volume6
Issue number1
DOIs
StatePublished - 2015

Keywords

  • Acinetobacter
  • Colitis
  • Inflammation
  • Microbiome
  • Mucosa
  • Proteobacteria

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