Induction of lysis by T cell receptor γδ+/CD3+ T lymphocytes via CD2 requires triggering via the T11.1 epitope only

P. S. Goedegebuure, D. M. Segal, E. Braakman, R. J. Vreugdenhil, B. A. Van Krimpen, R. J. Van de Griend, R. L.H. Bolhuis

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

The requirements for activation of the lytic machinery through CD2 of TCRγδ+/CD3+ cells were examined, by utilizing bispecific heteroconjugates containing anti-CD2 mAb cross-linked to anti-DNP. Contrary to the CD2 activation requirements in TCRαβ+/CD3+ cells, cytotoxic activity in TCRγδ+/CD3+ clones and TCR-/CD3- NK cell clones can be induced by heteroconjugates containing a single anti-CD2 (OKT11.1) mAb. Activation of TCRγδ+/CD3+ cells via CD2 is independent of heteroconjugate binding to CD16 (FcγRIII), because heteroconjugates prepared from Fab fragments induced equal levels of lysis. Moreover, anti-CD16 mAb did not inhibit triggering via CD2 in TCRγδ+/CD3+ cells. In TCR-/CD3- NK cells, however, induction of cytotoxicity via CD2 is co-dependent on interplay with CD16. Anti-CD3 mAb blocked the anti-CD2 x anti-DNP heteroconjugate-induced cytotoxicity of TCRγδ+/CD3+ cells, indicating a functional linkage between CD2 and CD3 on these cells. We conclude that induction of lysis via CD2 shows qualitatively different activation requirements in TCRγδ+/CD3+, TCRαβ+/CD3+ CTL and TCR-/CD3- NK cells.

Original languageEnglish
Pages (from-to)1797-1802
Number of pages6
JournalJournal of Immunology
Volume142
Issue number6
StatePublished - Jan 1 1989

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