TY - JOUR
T1 - Induction of lysis by T cell receptor γδ+/CD3+ T lymphocytes via CD2 requires triggering via the T11.1 epitope only
AU - Goedegebuure, P. S.
AU - Segal, D. M.
AU - Braakman, E.
AU - Vreugdenhil, R. J.
AU - Van Krimpen, B. A.
AU - Van de Griend, R. J.
AU - Bolhuis, R. L.H.
PY - 1989/1/1
Y1 - 1989/1/1
N2 - The requirements for activation of the lytic machinery through CD2 of TCRγδ+/CD3+ cells were examined, by utilizing bispecific heteroconjugates containing anti-CD2 mAb cross-linked to anti-DNP. Contrary to the CD2 activation requirements in TCRαβ+/CD3+ cells, cytotoxic activity in TCRγδ+/CD3+ clones and TCR-/CD3- NK cell clones can be induced by heteroconjugates containing a single anti-CD2 (OKT11.1) mAb. Activation of TCRγδ+/CD3+ cells via CD2 is independent of heteroconjugate binding to CD16 (FcγRIII), because heteroconjugates prepared from Fab fragments induced equal levels of lysis. Moreover, anti-CD16 mAb did not inhibit triggering via CD2 in TCRγδ+/CD3+ cells. In TCR-/CD3- NK cells, however, induction of cytotoxicity via CD2 is co-dependent on interplay with CD16. Anti-CD3 mAb blocked the anti-CD2 x anti-DNP heteroconjugate-induced cytotoxicity of TCRγδ+/CD3+ cells, indicating a functional linkage between CD2 and CD3 on these cells. We conclude that induction of lysis via CD2 shows qualitatively different activation requirements in TCRγδ+/CD3+, TCRαβ+/CD3+ CTL and TCR-/CD3- NK cells.
AB - The requirements for activation of the lytic machinery through CD2 of TCRγδ+/CD3+ cells were examined, by utilizing bispecific heteroconjugates containing anti-CD2 mAb cross-linked to anti-DNP. Contrary to the CD2 activation requirements in TCRαβ+/CD3+ cells, cytotoxic activity in TCRγδ+/CD3+ clones and TCR-/CD3- NK cell clones can be induced by heteroconjugates containing a single anti-CD2 (OKT11.1) mAb. Activation of TCRγδ+/CD3+ cells via CD2 is independent of heteroconjugate binding to CD16 (FcγRIII), because heteroconjugates prepared from Fab fragments induced equal levels of lysis. Moreover, anti-CD16 mAb did not inhibit triggering via CD2 in TCRγδ+/CD3+ cells. In TCR-/CD3- NK cells, however, induction of cytotoxicity via CD2 is co-dependent on interplay with CD16. Anti-CD3 mAb blocked the anti-CD2 x anti-DNP heteroconjugate-induced cytotoxicity of TCRγδ+/CD3+ cells, indicating a functional linkage between CD2 and CD3 on these cells. We conclude that induction of lysis via CD2 shows qualitatively different activation requirements in TCRγδ+/CD3+, TCRαβ+/CD3+ CTL and TCR-/CD3- NK cells.
UR - http://www.scopus.com/inward/record.url?scp=0024601123&partnerID=8YFLogxK
M3 - Article
C2 - 2466076
AN - SCOPUS:0024601123
SN - 0022-1767
VL - 142
SP - 1797
EP - 1802
JO - Journal of Immunology
JF - Journal of Immunology
IS - 6
ER -