Induction of cytocidal macrophages after in vitro interactions between Listeria-immune T cells and macrophages - role of H-2

A. G. Farr, W. J. Wechter, J. M. Kiely, E. R. Unanue

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Twenty-four hour cultures of macrophages from nonimmune mice with T cells from mice immune to Listeria resulted in the acquisition of cytocidal activity by the macrophages. The macrophages killed P815 tumor cells in a chromium-release assay. Macrophages from nonimmune mice given peptone or thioglycollate injections were much better effector cells than resident macrophages. Experiments established the conditions for generation of cytolytic macrophages: the specificity of T cells to Listeria and the need for macrophages to specifically interact with heat-killed Listeria organisms. Cells from the immune T cell inoculum did not participate in the killing process by the activated macrophages. The interactions leading to the generation of cytocidal macrophages required homology at the I region between macrophages and T cells, as well as the presence of macrophages bearing Ia molecules. Cultures of T cells and macrophages with heat-killed Listeria contained a factor that, when added to peptone macrophages, resulted in their activation for cytolytic activity. The factor could activate syngeneic or allogeneic macrophages, or macrophages lacking detectable Ia molecules. The immunogeneic moiety associated with Listeria was shortlived.

Original languageEnglish
Pages (from-to)2405-2412
Number of pages8
JournalJournal of Immunology
Volume122
Issue number6
StatePublished - Dec 1 1979

Fingerprint

Dive into the research topics of 'Induction of cytocidal macrophages after in vitro interactions between Listeria-immune T cells and macrophages - role of H-2'. Together they form a unique fingerprint.

Cite this