@article{c8f2af898fd347078e1f77153b2a69f2,
title = "Induction of a torpor-like hypothermic and hypometabolic state in rodents by ultrasound",
abstract = "Torpor is an energy-conserving state in which animals dramatically decrease their metabolic rate and body temperature to survive harsh environmental conditions. Here, we report the noninvasive, precise and safe induction of a torpor-like hypothermic and hypometabolic state in rodents by remote transcranial ultrasound stimulation at the hypothalamus preoptic area (POA). We achieve a long-lasting (>24 h) torpor-like state in mice via closed-loop feedback control of ultrasound stimulation with automated detection of body temperature. Ultrasound-induced hypothermia and hypometabolism (UIH) is triggered by activation of POA neurons, involves the dorsomedial hypothalamus as a downstream brain region and subsequent inhibition of thermogenic brown adipose tissue. Single-nucleus RNA-sequencing of POA neurons reveals TRPM2 as an ultrasound-sensitive ion channel, the knockdown of which suppresses UIH. We also demonstrate that UIH is feasible in a non-torpid animal, the rat. Our findings establish UIH as a promising technology for the noninvasive and safe induction of a torpor-like state.",
author = "Yaoheng Yang and Jinyun Yuan and Field, {Rachael L.} and Dezhuang Ye and Zhongtao Hu and Kevin Xu and Lu Xu and Yan Gong and Yimei Yue and Kravitz, {Alexxai V.} and Bruchas, {Michael R.} and Jianmin Cui and Brestoff, {Jonathan R.} and Hong Chen",
note = "Funding Information: We acknowledge J. D. Quirk for helping with the technical issue of the MRI scanner. We thank L. Li from the University of Washington, C.-K. Mo and X. Liu for providing us with valuable suggestions on the single-nucleus RNA-seq data analysis. We also thank A. Norris and A. Cone for helping us setting up body temperature imaging using thermal camera. We thank M. Rhodes for helping with imaging the FISH-stained slices. Some of the cartoons were created with BioRender.com. This work was supported by the NIH R01MH116981 (H.C.), UG3MH126861 (H.C.), R01EB027223 (H.C.) and R01EB030102 (H.C.). J.R.B. is supported by NIH DP5 OD028125 and Burroughs Wellcome Fund CAMS no. 1019648. Funding Information: We acknowledge J. D. Quirk for helping with the technical issue of the MRI scanner. We thank L. Li from the University of Washington, C.-K. Mo and X. Liu for providing us with valuable suggestions on the single-nucleus RNA-seq data analysis. We also thank A. Norris and A. Cone for helping us setting up body temperature imaging using thermal camera. We thank M. Rhodes for helping with imaging the FISH-stained slices. Some of the cartoons were created with BioRender.com. This work was supported by the NIH R01MH116981 (H.C.), UG3MH126861 (H.C.), R01EB027223 (H.C.) and R01EB030102 (H.C.). J.R.B. is supported by NIH DP5 OD028125 and Burroughs Wellcome Fund CAMS no. 1019648. Publisher Copyright: {\textcopyright} 2023, The Author(s).",
year = "2023",
month = may,
doi = "10.1038/s42255-023-00804-z",
language = "English",
volume = "5",
pages = "789--803",
journal = "Nature Metabolism",
issn = "2522-5812",
number = "5",
}