TY - JOUR
T1 - Induction of a nerve growth factor-sensitive kinase that phosphorylates the DNA-binding domain of the orphan nuclear receptor NGFI-B
AU - Hirata, Yoko
AU - Whalin, Michael
AU - Ginty, David D.
AU - Xing, Jun
AU - Greenberg, Michael E.
AU - Milbrandt, Jeffrey
AU - Guroff, Gordon
PY - 1995/10
Y1 - 1995/10
N2 - Nerve growth factor (NGF) induces the synthesis and the phosphorylation of the orphan nuclear receptor NGFI-B in PC12 cells. Previous work has shown that phosphorylation, by protein kinase A, of a specific serine in the DNA- binding domain inhibits its binding to the NGFI-B response element. Also, cytoplasmic extracts from PC12 cells phosphorylate this serine, and phosphorylation is greater in extracts from cells treated with NGF. The present work describes the induction, identification, and partial purification of a kinase (termed NGFI-B kinase I) from PC12 cell extracts that catalyzes this phosphorylation. Phosphorylation of the DNA-binding domain with this purified preparation inhibits its binding to the NGFI-B response element. The kinase is rapidly activated by treatment of the cells with NGF, and the activation lasts for at least several hours. It also is activated by fibroblast growth factor and epidermal growth factor (EGF), but the activation by EGF is quite transient. The kinase requires Mg2+ but will use Mn2+. The molecular mass of the kinase is 95-100 kDa, and it is different from protein kinase A, Fos kinase, or pp90(rsk). Comparison with a partially purified preparation of cyclic AMP response element-binding protein kinase, however, indicates that the two are either very similar or identical.
AB - Nerve growth factor (NGF) induces the synthesis and the phosphorylation of the orphan nuclear receptor NGFI-B in PC12 cells. Previous work has shown that phosphorylation, by protein kinase A, of a specific serine in the DNA- binding domain inhibits its binding to the NGFI-B response element. Also, cytoplasmic extracts from PC12 cells phosphorylate this serine, and phosphorylation is greater in extracts from cells treated with NGF. The present work describes the induction, identification, and partial purification of a kinase (termed NGFI-B kinase I) from PC12 cell extracts that catalyzes this phosphorylation. Phosphorylation of the DNA-binding domain with this purified preparation inhibits its binding to the NGFI-B response element. The kinase is rapidly activated by treatment of the cells with NGF, and the activation lasts for at least several hours. It also is activated by fibroblast growth factor and epidermal growth factor (EGF), but the activation by EGF is quite transient. The kinase requires Mg2+ but will use Mn2+. The molecular mass of the kinase is 95-100 kDa, and it is different from protein kinase A, Fos kinase, or pp90(rsk). Comparison with a partially purified preparation of cyclic AMP response element-binding protein kinase, however, indicates that the two are either very similar or identical.
KW - Kinase
KW - NGFI-B
KW - Nerve growth factor
KW - PC12 cells
UR - http://www.scopus.com/inward/record.url?scp=0029154338&partnerID=8YFLogxK
M3 - Article
C2 - 7561876
AN - SCOPUS:0029154338
SN - 0022-3042
VL - 65
SP - 1780
EP - 1788
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 4
ER -