Inducible nitric oxide synthase gene expression and enzyme activity correlate with disease activity in murine experimental autoimmune encephalomyelitis

Anne H. Cross, Richard M. Keeling, Salil Goorha, Manuel San, Charles Rodi, Pamela S. Wyatt, Pamela T. Manning, Thomas P. Misko

Research output: Contribution to journalArticlepeer-review

86 Scopus citations

Abstract

Messenger RNA encoding inducible NO synthase (iNOS) was measured by competitive reverse transcriptase polymerase chain reaction (cRT-PCR) and ribonuclease protection assays in spinal cords from mice at varying stages of experimental allergic encephalomyelitis (EAE) and from control mice. iNOS mRNA was increased in spinal cords from mice with acute EAE. cRT-PCR assays revealed a 10-20-fold increase in iNOS mRNA in spinal cords during acute EAE compared with the level observed in normal mouse spinal cords. Functional iNOS activity, as assessed by assay of calcium-independent citrulline production, was also significantly increased in spinal cords from mice with acute EAE in comparison to normal controls. The correlation of functional iNOS expression with active disease in EAE is consistent with a pathogenic role for excess NO in this model of cell-mediated central nervous system autoimmunity.

Original languageEnglish
Pages (from-to)145-153
Number of pages9
JournalJournal of Neuroimmunology
Volume71
Issue number1-2
DOIs
StatePublished - Dec 1996

Keywords

  • competitive reverse transcriptase PCR
  • experimental allergic (autoimmune) encephalomyelitis
  • nitric oxide

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