Independently melting modules and highly structured intermodular junctions within complement receptor type 1

Marina D. Kirkitadze, Malgorzata Krych, Dusan Uhrin, David T.F. Dryden, Brian O. Smith, Alan Cooper, Xuefeng Wang, Richard Hauhart, John P. Atkinson, Paul N. Barlow

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

A segment of complement receptor type 1 (CR1) corresponding to modules 15-17 was overexpressed as a functionally active recombinant protein with N- glycosylation sites ablated by mutagenesis (referred to as CR1~15-17-). A protein consisting of modules 15 and 16 and another corresponding to module 16 were also overexpressed. Comparison of heteronuclear nuclear magnetic resonance (NMR) spectra for the single, double, and triple module fragments indicated that module 16 makes more extensive contacts with module 15 than with module 17. A combination of NMR, differential scanning calorimetry, circular dichroism, and tryptophan-derived fluorescence indicated a complex unfolding pathway for CR1~15-17-. As temperature or denaturant concentration was increased, the 16-17 junction appeared to melt first, followed by the 15-16 junction, and module 17 itself; finally, modules 15 and 16 became denatured. Modules 15 and 16 adopted an intermediate state prior to total denaturation. These results are compared with a previously published study [Clark, N. S., Dodd, I, Mossakowska, D. E., Smith, R. A. G., and Gore, M. G. (1996) Protein Eng. 9, 877-884] on a fragment consisting of the N- terminal three CR1 modules which appeared to melt as a single unit.

Original languageEnglish
Pages (from-to)7019-7031
Number of pages13
JournalBiochemistry
Volume38
Issue number22
DOIs
StatePublished - Jun 1 1999

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