TY - JOUR
T1 - Independent selection by I-A(k) molecules of two epitopes found in tandem in an extended polypeptide antigen
AU - Gugasyan, R.
AU - Velazquez, C.
AU - Vidavsky, I.
AU - Deck, B. M.
AU - Van der Drift, K.
AU - Gross, M. L.
AU - Unanue, E. R.
PY - 2000/9/15
Y1 - 2000/9/15
N2 - The protein hen egg white lysozyme (HEL) contains two segments, in tandem, from which two families of peptides are selected by the class II molecule I-A(k), during processing. These encompass peptides primarily from residues 31-47 and 48-63. Mutant HEL proteins were created with changes in residues 52 and 55, resulting in a lack of binding and selection of the 48-63 peptides to I-A(k) molecules. Such mutant HEL proteins donated the same amount of 31-47 peptide as did the modified protein. Other mutant HEL molecules containing proline residues at residue 46, 47, or 48 resulted in extensions of the selected 31-47 or 48-62 families to their overlapping regions (in the carboxyl or amino termini, respectively). However, the amount of each family of peptide selected was not changed. We conclude that the presence or absence of the major peptide from HEL does not influence the selection of other epitopes, and that these two families are selected independently of each other.
AB - The protein hen egg white lysozyme (HEL) contains two segments, in tandem, from which two families of peptides are selected by the class II molecule I-A(k), during processing. These encompass peptides primarily from residues 31-47 and 48-63. Mutant HEL proteins were created with changes in residues 52 and 55, resulting in a lack of binding and selection of the 48-63 peptides to I-A(k) molecules. Such mutant HEL proteins donated the same amount of 31-47 peptide as did the modified protein. Other mutant HEL molecules containing proline residues at residue 46, 47, or 48 resulted in extensions of the selected 31-47 or 48-62 families to their overlapping regions (in the carboxyl or amino termini, respectively). However, the amount of each family of peptide selected was not changed. We conclude that the presence or absence of the major peptide from HEL does not influence the selection of other epitopes, and that these two families are selected independently of each other.
UR - http://www.scopus.com/inward/record.url?scp=0034665098&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.165.6.3206
DO - 10.4049/jimmunol.165.6.3206
M3 - Article
C2 - 10975836
AN - SCOPUS:0034665098
SN - 0022-1767
VL - 165
SP - 3206
EP - 3213
JO - Journal of Immunology
JF - Journal of Immunology
IS - 6
ER -