Inactivation of NADPH oxidase organizer 1 results in severe imbalance

Péter J. Kiss, Judit Knisz, Yuzhou Zhang, Jonas Baltrusaitis, Curt D. Sigmund, Ruediger Thalmann, Richard J.H. Smith, Elisabeth Verpy, Botond Bánfi

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

Otoconia are biominerals of the vestibular system that are indispensable for the perception of gravity. Despite their importance, the process of otoconia genesis is largely unknown. Reactive oxygen species (ROS) have been recognized for their toxic effects in antimicrobial host defense as well as in aging and carcinogenesis. Enzymes evolved for ROS production belong to the recently discovered NADPH oxidase (Nox) enzyme family [1]. Here we show that the inactivation of a regulatory subunit, NADPH oxidase organizer 1 (Noxo1), resulted in the severe balance deficit seen in the spontaneous mutant "head slant" (hslt) mice whose phenotype was rescued by Noxo1 transgenes. Wild-type Noxo1 was expressed in the vestibular and cochlear epithelia and was required for ROS production by an oxidase complex. In contrast, the hslt mutation of Noxo1 was biochemically inactive and led to an arrest of otoconia genesis, characterized by a complete lack of calcium carbonate mineralization and an accumulation of otoconial protein, otoconin-90/95 (OC-90/95). These results suggest that ROS generated by a Noxo1-dependent vestibular oxidase are critical for otoconia formation and may be required for interactions among otoconial components. Noxo1 mutants implicate a constructive developmental role for ROS, in contrast to their previously described toxic effects.

Original languageEnglish
Pages (from-to)208-213
Number of pages6
JournalCurrent Biology
Volume16
Issue number2
DOIs
StatePublished - Jan 24 2006

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    Kiss, P. J., Knisz, J., Zhang, Y., Baltrusaitis, J., Sigmund, C. D., Thalmann, R., Smith, R. J. H., Verpy, E., & Bánfi, B. (2006). Inactivation of NADPH oxidase organizer 1 results in severe imbalance. Current Biology, 16(2), 208-213. https://doi.org/10.1016/j.cub.2005.12.025