Inactivation of BK channels mediated by the NH2 terminus of the β3b auxiliary subunit involves a two-step mechanism: Possible separation of binding and blockade

C. J. Lingle, X. H. Zeng, J. P. Ding, X. M. Xia

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

A family of auxiliary β subunits coassemble with Slo α subunit to form Ca2+-regulated, voltage-activated BK-type K+ channels. The β subunits play an important role in regulating the functional properties of the resulting channel protein, including apparent Ca2+ dependence and inactivation. The β3b auxiliary subunit, when coexpressed with the Slo α subunit, results in a particularly rapid (∼1 ms), but incomplete inactivation, mediated by the cytosolic NH2 terminus of the β3b subunit (Xia et al., 2000). Here, we evaluate whether a simple block of the open channel by the NH2-terminal domain accounts for the inactivation mechanism. Analysis of the onset of block, recovery from block, time-dependent changes in the shape of instantaneous current-voltage curves, and properties of deactivation tails suggest that a simple, one step blocking reaction is insufficient to explain the observed currents. Rather, blockade can be largely accounted for by a two-step blocking mechanism (Cn ⇌ On ⇌ On ⇌ In) in which preblocked open states (O*n) precede blocked states (In). The transitions between O* and I are exceedingly rapid accounting for an almost instantaneous block or unblock of open channels observed with changes in potential. However, the macroscopic current relaxations are determined primarily by slower transitions between O and O*. We propose that the O to O* transition corresponds to binding of the NH2-terminal inactivation domain to a receptor site. Blockade of current subsequently reflects either additional movement of the NH2-terminal domain into a position that hinders ion permeation or a gating transition to a closed state induced by binding of the NH2 terminus.

Original languageEnglish
Pages (from-to)583-605
Number of pages23
JournalJournal of General Physiology
Volume117
Issue number6
DOIs
StatePublished - Jul 3 2001

Keywords

  • Ca-and voltage-gated K channels
  • Channel block
  • Gating mechanisms
  • K channels
  • mSlo channels

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