@inproceedings{699441ffb4e248f9beea52f1bf9b0db3,
title = "In vivo three-dimensional superresolution fluorescence tracking using a double-helix point spread function",
abstract = "The point spread function (PSF) of a widefield fluorescence microscope is not suitable for three-dimensional superresolution imaging. We characterize the localization precision of a unique method for 3D superresolution imaging featuring a double-helix point spread function (DH-PSF). The DH-PSF is designed to have two lobes that rotate about their midpoint in any transverse plane as a function of the axial position of the emitter. In effect, the PSF appears as a double helix in three dimensions. By comparing the Cramer-Rao bound of the DH-PSF with the standard PSF as a function of the axial position, we show that the DH-PSF has a higher and more uniform localization precision than the standard PSF throughout a 2 μm depth of field. Comparisons between the DH-PSF and other methods for 3D superresolution are briefly discussed. We also illustrate the applicability of the DH-PSF for imaging weak emitters in biological systems by tracking the movement of quantum dots in glycerol and in live cells.",
keywords = "Fluorescence microscopy, Quantum dot, Single-particle tracking, Superresolution, Three-dimensional microscopy",
author = "Lew, \{Matthew D.\} and Thompson, \{Michael A.\} and Majid Badieirostami and Moerner, \{W. E.\}",
year = "2010",
doi = "10.1117/12.842608",
language = "English",
isbn = "9780819479679",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Single Molecule Spectroscopy and Imaging III",
note = "Single Molecule Spectroscopy and Imaging III ; Conference date: 23-01-2010 Through 25-01-2010",
}