TY - JOUR
T1 - In vivo role of myeloperoxidase for the host defense
AU - Aratani, Yasuaki
AU - Kura, Fumiaki
AU - Watanabe, Haruo
AU - Akagawa, Hisayoshi
AU - Takano, Yukie
AU - Suzuki, Kazuo
AU - Dinauer, Mary C.
AU - Maeda, Nobuyo
AU - Koyama, Hideki
PY - 2004/10/1
Y1 - 2004/10/1
N2 - Myeloperoxidase (MPO) is located within neutrophils capable of producing HOC1. To define the in vivo role of MPO, we have generated MPO-knockout (MPO-KO) mice. The mice without MPO developed normally. However, MPO-KO mice showed severely reduced cytotoxicity to various microorganisms such as Candida albicans, Aspergillus fumigatus, and Klebsiella pneumoniae, demonstrating that MPO-dependent oxidative system is important for host defense against fungi and bacteria, although the effect varies from species to species of pathogens. To compare the importance of MPO and NADPH-oxidase for host defense, MPO-KO and chronic granulomatous disease (CGD) mice were infected with different doses of C. albicans, and their infection severity was analyzed. CGD mice exhibited increased mortality and tissue fungal burden in a dose-dependent manner, whereas normal mice showed no symptoms. Interestingly, at the highest dose, the mortality of MPO-KO mice was comparable to CGD mice, but was the same as normal mice at the lowest dose. These results suggest that MPO and NADPH-oxidase are equally important for early host defense against a large inocula of Candida.
AB - Myeloperoxidase (MPO) is located within neutrophils capable of producing HOC1. To define the in vivo role of MPO, we have generated MPO-knockout (MPO-KO) mice. The mice without MPO developed normally. However, MPO-KO mice showed severely reduced cytotoxicity to various microorganisms such as Candida albicans, Aspergillus fumigatus, and Klebsiella pneumoniae, demonstrating that MPO-dependent oxidative system is important for host defense against fungi and bacteria, although the effect varies from species to species of pathogens. To compare the importance of MPO and NADPH-oxidase for host defense, MPO-KO and chronic granulomatous disease (CGD) mice were infected with different doses of C. albicans, and their infection severity was analyzed. CGD mice exhibited increased mortality and tissue fungal burden in a dose-dependent manner, whereas normal mice showed no symptoms. Interestingly, at the highest dose, the mortality of MPO-KO mice was comparable to CGD mice, but was the same as normal mice at the lowest dose. These results suggest that MPO and NADPH-oxidase are equally important for early host defense against a large inocula of Candida.
UR - http://www.scopus.com/inward/record.url?scp=9144241697&partnerID=8YFLogxK
M3 - Article
C2 - 15507755
AN - SCOPUS:9144241697
VL - 57
SP - S15
JO - Japanese Journal of Infectious Diseases
JF - Japanese Journal of Infectious Diseases
SN - 1344-6304
IS - 5
ER -