In vivo role of myeloperoxidase for the host defense

Yasuaki Aratani, Fumiaki Kura, Haruo Watanabe, Hisayoshi Akagawa, Yukie Takano, Kazuo Suzuki, Mary C. Dinauer, Nobuyo Maeda, Hideki Koyama

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16 Scopus citations

Abstract

Myeloperoxidase (MPO) is located within neutrophils capable of producing HOC1. To define the in vivo role of MPO, we have generated MPO-knockout (MPO-KO) mice. The mice without MPO developed normally. However, MPO-KO mice showed severely reduced cytotoxicity to various microorganisms such as Candida albicans, Aspergillus fumigatus, and Klebsiella pneumoniae, demonstrating that MPO-dependent oxidative system is important for host defense against fungi and bacteria, although the effect varies from species to species of pathogens. To compare the importance of MPO and NADPH-oxidase for host defense, MPO-KO and chronic granulomatous disease (CGD) mice were infected with different doses of C. albicans, and their infection severity was analyzed. CGD mice exhibited increased mortality and tissue fungal burden in a dose-dependent manner, whereas normal mice showed no symptoms. Interestingly, at the highest dose, the mortality of MPO-KO mice was comparable to CGD mice, but was the same as normal mice at the lowest dose. These results suggest that MPO and NADPH-oxidase are equally important for early host defense against a large inocula of Candida.

Original languageEnglish
Pages (from-to)S15
JournalJapanese Journal of Infectious Diseases
Volume57
Issue number5
StatePublished - Oct 1 2004
Externally publishedYes

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    Aratani, Y., Kura, F., Watanabe, H., Akagawa, H., Takano, Y., Suzuki, K., Dinauer, M. C., Maeda, N., & Koyama, H. (2004). In vivo role of myeloperoxidase for the host defense. Japanese Journal of Infectious Diseases, 57(5), S15.