In vivo MRS measurement of liver lipid levels in mice

J. R. Garbow, X. Lin, N. Sakata, Z. Chen, D. Koh, G. Schonfeld

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

A magnetic resonance spectroscopy (MRS) procedure for in vivo measurement of lipid levels in mouse liver is described and validated. The method uses respiratory-gated, localized spectroscopy to collect proton spectra from voxels within the mouse liver. Bayesian probability theory analysis of these spectra allows the relative intensifies of the lipid and water resonances within the liver to be accurately measured. All spectral data were corrected for measured spin-spin relaxation. A total of 48 mice were used in this study, including wild-type mice and two different transgenic mouse strains. Different groups of these mice were fed high-fat or low-fat diets or liquid diets with and without the addition of alcohol. Proton spectra were collected at baseline and, subsequently, every 4 weeks for up to 16 weeks. Immediately after the last MRS measurement, mice were killed and their livers analyzed for triglyceride level by conventional wet-chemistry methods. The excellent correlation between in vivo MRS and ex vivo wet-chemistry determinations of liver lipids validates the MRS method. These results clearly demonstrate that in vivo MRS will be an extremely valuable technique for longitudinal studies aimed at providing important insights into the genetic, environmental, and dietary factors affecting fat deposition and accumulation within the mouse liver.

Original languageEnglish
Pages (from-to)1364-1371
Number of pages8
JournalJournal of lipid research
Volume45
Issue number7
DOIs
StatePublished - Jul 2004

Keywords

  • Cirrhosis
  • Fatty liver
  • Magnetic resonance spectroscopy
  • Triglyceride

Fingerprint

Dive into the research topics of 'In vivo MRS measurement of liver lipid levels in mice'. Together they form a unique fingerprint.

Cite this