In vivo measurement of normal rat intracellular pyruvate and lactate levels after injection of hyperpolarized [1- 13C]alanine

Simon Hu, Minhua Zhu, Hikari A.I. Yoshihara, David M. Wilson, Kayvan R. Keshari, Peter Shin, Galen Reed, Cornelius von Morze, Robert Bok, Peder E.Z. Larson, John Kurhanewicz, Daniel B. Vigneron

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Hyperpolarized technology utilizing dynamic nuclear polarization has enabled rapid and high-sensitivity measurements of 13C metabolism in vivo. The most commonly used in vivo agent for hyperpolarized 13C metabolic imaging thus far has been [1- 13C]pyruvate. In preclinical studies, not only is its uptake detected, but also its intracellular enzymatic conversion to metabolic products including [1- 13C]lactate and [1- 13C]alanine. However, the ratio of 13C-lactate/ 13C-pyruvate measured in this data does not accurately reflect cellular values since much of the [1- 13C]pyruvate is extracellular depending on timing, vascular properties, and extracellular space and monocarboxylate transporter activity. In order to measure the relative levels of intracellular pyruvate and lactate, in this project we hyperpolarized [1- 13C]alanine and monitored the in vivo conversion to [1- 13C]pyruvate and then the subsequent conversion to [1- 13C]lactate. The intracellular lactate-to-pyruvate ratio of normal rat tissue measured with hyperpolarized [1- 13C]alanine was 4.89±0.61 (mean±S.E.) as opposed to a ratio of 0.41±0.03 when hyperpolarized [1- 13C]pyruvate was injected.

Original languageEnglish
Pages (from-to)1035-1040
Number of pages6
JournalMagnetic Resonance Imaging
Volume29
Issue number8
DOIs
StatePublished - Oct 2011

Keywords

  • Dynamic nuclear polarization (DNP)
  • Hyperpolarized carbon-13
  • Magnetic resonance spectroscopy/spectroscopic imaging
  • Monocarboxylate transporter (MCT)

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