In vivo label-free photoacoustic microscopy of the anterior segment of the mouse eye

Bin Rao, Song Hu, Li Li, Konstantin Maslov, Lihong V. Wang

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

5 Scopus citations

Abstract

Both iris fluorescein angiography (IFA) and indocyanine green angiography (ICGA) provide ophthalmologists imaging tools in studying the microvasculature structure and hemodynamics of the anterior segment of the eye in normal and diseased status. However, a non-invasive, endogenous imaging modality is preferable for the monitoring of hemodynamics of the iris microvasculature. We investigated the in vivo, label-free ocular anterior segment imaging with photo-acoustic microscopy (PAM) in mouse eyes. We demonstrated the unique advantage of endogenous contrast that is not available in both IFA and ICGA. The laser radiation was maintained within the ANSI laser safety limit. The in vivo, label-free nature of our imaging technology has the potential for ophthalmic applications.

Original languageEnglish
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2010
PublisherSPIE
ISBN (Print)9780819479600
DOIs
StatePublished - Feb 11 2010
EventPhotons Plus Ultrasound: Imaging and Sensing 2010 - San Francisco, CA, United States
Duration: Jan 24 2010Jan 26 2010

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7564
ISSN (Print)1605-7422

Conference

ConferencePhotons Plus Ultrasound: Imaging and Sensing 2010
Country/TerritoryUnited States
CitySan Francisco, CA
Period01/24/1001/26/10

Keywords

  • Anterior segment of eye
  • Iris imaging
  • Photoacoustic microscopy

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