In vivo investigation of pharmacokinetics of model drug: Comparison of near infrared technique with high-performance liquid chromatography

Yueqing Gu, Fei Liu, Chunsheng Fang, Zhiyu Qian, Samuel Achilefu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Near infrared spectroscopy possess great potential for in vivo quantitative monitoring of drugs in animal subject. The accuracy of the measurements by near infrared technique should be evaluated by an established method. In this study, a near infrared fluorescence dye, cypate and its conjugation cypate-PEG were used as model drug for in vivo dynamic study. The pharmacokinetics of the model drug in mice subjects were investigated by near infrared spectroscopy and high performance liquid chromatography, respectively. The results from the two techniques were compared. The pharmacokinetic parameters calculated based on the acquired data by DAS software showed that there were no statistical differences between the two methods. The dynamic distribution of the model drugs in mouse model imaged by NIR image system indicated that cypate firstly accumulated in liver and was cleared from the enteron system, while cypate - PEG clearance from the urine system. Results indicated that NIR monitoring technique provide a promising quantitative way for in vivo monitoring the dynamics of drugs in animal subjects.

Original languageEnglish
Title of host publicationReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II
DOIs
StatePublished - May 10 2010
EventReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II - San Francisco, CA, United States
Duration: Jan 25 2010Jan 27 2010

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7576
ISSN (Print)1605-7422

Conference

ConferenceReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II
CountryUnited States
CitySan Francisco, CA
Period01/25/1001/27/10

Keywords

  • Dynamic distribution
  • Fluorescence measurement
  • HPLC
  • Near-infrared technique
  • Pharmacokinetics

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  • Cite this

    Gu, Y., Liu, F., Fang, C., Qian, Z., & Achilefu, S. (2010). In vivo investigation of pharmacokinetics of model drug: Comparison of near infrared technique with high-performance liquid chromatography. In Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II [75760A] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7576). https://doi.org/10.1117/12.840332