In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure

Yu Wang; Song Hu; Konstantin Maslov; Yu Zhang; Younan Xia; Lihong V. Wang

doi:10.1364/OL.36.001029

In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure

Yu Wang, Song Hu, Konstantin Maslov, Yu Zhang, Younan Xia, Lihong V. Wang

Department of Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

115 Scopus citations

Abstract

We developed dual-modality microscope integrating photoacoustic microscopy (PAM) and fluorescence confocal microscopy (FCM) to noninvasively image hemoglobin oxygen saturation (sO₂) and oxygen partial pressure (pO₂) in vivo in single blood vessels with high spatial resolution. While PAM measures sO₂ by imaging hemoglobin optical absorption at two wavelengths, FCM quantifies pO₂ using phosphorescence quenching. The variations of sO₂ and pO₂ values in multiple orders of vessel branches under hyperoxic (100% oxygen) and normoxic (21% oxygen) conditions correlate well with the oxygen-hemoglobin dissociation curve. In addition, the total concentration of hemoglobin is imaged by PAM at an isosbestic wavelength.

Original language	English
Pages (from-to)	1029-1031
Number of pages	3
Journal	Optics Letters
Volume	36
Issue number	7
DOIs	https://doi.org/10.1364/OL.36.001029
State	Published - Apr 1 2011

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title = "In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure",

abstract = "We developed dual-modality microscope integrating photoacoustic microscopy (PAM) and fluorescence confocal microscopy (FCM) to noninvasively image hemoglobin oxygen saturation (sO2) and oxygen partial pressure (pO2) in vivo in single blood vessels with high spatial resolution. While PAM measures sO2 by imaging hemoglobin optical absorption at two wavelengths, FCM quantifies pO2 using phosphorescence quenching. The variations of sO2 and pO2 values in multiple orders of vessel branches under hyperoxic (100\% oxygen) and normoxic (21\% oxygen) conditions correlate well with the oxygen-hemoglobin dissociation curve. In addition, the total concentration of hemoglobin is imaged by PAM at an isosbestic wavelength.",

author = "Yu Wang and Song Hu and Konstantin Maslov and Yu Zhang and Younan Xia and Wang, \{Lihong V.\}",

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T1 - In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure

AU - Wang, Yu

AU - Hu, Song

AU - Maslov, Konstantin

AU - Zhang, Yu

AU - Xia, Younan

AU - Wang, Lihong V.

PY - 2011/4/1

Y1 - 2011/4/1

N2 - We developed dual-modality microscope integrating photoacoustic microscopy (PAM) and fluorescence confocal microscopy (FCM) to noninvasively image hemoglobin oxygen saturation (sO2) and oxygen partial pressure (pO2) in vivo in single blood vessels with high spatial resolution. While PAM measures sO2 by imaging hemoglobin optical absorption at two wavelengths, FCM quantifies pO2 using phosphorescence quenching. The variations of sO2 and pO2 values in multiple orders of vessel branches under hyperoxic (100% oxygen) and normoxic (21% oxygen) conditions correlate well with the oxygen-hemoglobin dissociation curve. In addition, the total concentration of hemoglobin is imaged by PAM at an isosbestic wavelength.

AB - We developed dual-modality microscope integrating photoacoustic microscopy (PAM) and fluorescence confocal microscopy (FCM) to noninvasively image hemoglobin oxygen saturation (sO2) and oxygen partial pressure (pO2) in vivo in single blood vessels with high spatial resolution. While PAM measures sO2 by imaging hemoglobin optical absorption at two wavelengths, FCM quantifies pO2 using phosphorescence quenching. The variations of sO2 and pO2 values in multiple orders of vessel branches under hyperoxic (100% oxygen) and normoxic (21% oxygen) conditions correlate well with the oxygen-hemoglobin dissociation curve. In addition, the total concentration of hemoglobin is imaged by PAM at an isosbestic wavelength.

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DO - 10.1364/OL.36.001029

M3 - Article

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SN - 0146-9592

VL - 36

SP - 1029

EP - 1031

JO - Optics Letters

JF - Optics Letters

IS - 7

ER -

In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure

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