@article{06341bcfb5fe44a5bb74954dfee4d1fc,
title = "In vivo detection of optically-evoked opioid peptide release",
abstract = "Though the last decade has seen accelerated advances in techniques and technologies to perturb neuronal circuitry in the brain, we are still poorly equipped to adequately dissect endogenous peptide release in vivo. To this end we developed a system that combines in vivo optogenetics with microdialysis and a highly sensitive mass spectrometry-based assay to measure opioid peptide release in freely moving rodents.",
author = "Ream Al-Hasani and Wong, {Jenny Marie T.} and Mabrouk, {Omar S.} and McCall, {Jordan G.} and Schmitz, {Gavin P.} and Porter-Stransky, {Kirsten A.} and Aragona, {Brandon J.} and Kennedy, {Robert T.} and Bruchas, {Michael R.}",
note = "Funding Information: This work is supported by NIDA R01 DA033396 (MRB), NIDA K99 DA038725 (RA), NIMH F31 MH101956 (JGM), WUSTL DBBS (JGM) and NIBIB R01 EB003320 (RTK). We thank the Bruchas Laboratory and Kennedy Laboratory for valuations discussions and support. We especially thank Shanna Resendez and Curtis Austin from the Aragona Laboratory for helpful discussion and technical assistance. We thank Karl Deisseroth (Stanford) for the channelrhodopsin-2 (H134) construct, Bradford Lowell (Harvard) and Michael Krashes (NIDDK) for the preprodynorphin-IRES-cre mice. We also thank The WUSTL HOPE Center viral vector core (NINDS, P30NS057105) and the University of Michigan Center for Statistical Consultation and Research (CSCAR, consultant Yumeng Li) and Luke Ziolkowski from the McCall Laboratory at Washington University who assisted with statistical analysis. Funding Information: This work is supported by NIDA R01 DA033396 (MRB), NIDA K99 DA038725 (RA), NIMH F31 MH101956 (JGM), WUSTL DBBS (JGM) and NIBIB R01 EB003320 (RTK). We thank the Bruchas Laboratory and Kennedy Laboratory for valuations discussions and support. We especially thank Shanna Resendez and Curtis Austin from the Aragona Laboratory for helpful discussion and technical assistance. We thank Karl Deisseroth (Stanford) for the channelrhodopsin-2 (H134) construct, Bradford Lowell (Harvard) and Michael Krashes (NIDDK) for the preprodynorphin-IRES-cre mice. We also thank The WUSTL HOPE Center viral vector core (NINDS, P30NS057105) and the University of Michigan Center for Statistical Consultation and Research (CSCAR, consultant Yumeng Li) and Luke Ziolkowski from the McCall Laboratory at Washington University who assisted with statistical analysis.Funder Grant reference number Author National Institute on Drug Abuse K99/R00 Pathway to Independence DA038725 Ream Al-Hasani National Institute of Mental Health F31 MH101956 Jordan G McCall National Institute of Biomedical Imaging and Bioengineering R01 EB003320 Robert T Kennedy National Institute on Drug Abuse R01 DA033396 Michael R Bruchas The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. Publisher Copyright: {\textcopyright} Al-Hasani et al.",
year = "2018",
month = sep,
doi = "10.7554/eLife.36520",
language = "English",
volume = "7",
journal = "eLife",
issn = "2050-084X",
}