In vivo detection of cervical dysplasia with near infrared Raman spectroscopy

Amy Robichaux, Chad Lieber, Heidi Shappell, Beth Huff, Howard Jones, Anita Mahadevan-Jansen

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Raman spectroscopy has been shown to have the potential for providing differential diagnosis in the cervix with high sensitivity and specificity in previous in vitro and in vivo studies. A clinical study was designed at Vanderbilt University Medical Center to further evaluate the potential of near infrared Raman spectroscopy for in vivo detection of squamous intra-epithelial neoplasia, a pre-cursor to cervical cancer, in a clinical setting. In this pilot in vivo clinical study, using a portable system, Raman spectra are collected using clinically feasible integration times (5-20 seconds) during colposcopic evaluation. Multiple Raman spectra were acquired from colposcopically normal and abnormal sites prior to excision of tissue from patients with known abnormalities of the cervix. Measured Raman spectra were processed for noise and background fluorescence using novel signal processing techniques. The resulting spectra were correlated with the corresponding histological diagnosis to determine empirical differences in spectra between different diagnostic categories. Using histology as the gold standard, multivariate statistical techniques were also used to develop discrimination algorithms with the hopes of developing this technique into a real time, non-invasive diagnostic tool.

Original languageEnglish
Pages (from-to)145-151
Number of pages7
JournalProceedings of SPIE - The International Society for Optical Engineering
Issue number1
StatePublished - Mar 22 2002


Dive into the research topics of 'In vivo detection of cervical dysplasia with near infrared Raman spectroscopy'. Together they form a unique fingerprint.

Cite this