In vivo depletion of mannose receptor bearing cells from rat liver by ricin A chain: Effects on clearance of β-glucuronidase

Barbara M. Simmons, Philip D. Stahl, John H. Russell

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Ricin A chain has previously been shown to intoxicate macrophages in vitro following binding and endocytosis by the macrophage mannose receptor. In this report it is demonstrated that the intravenous injection of ricin A chain in nephrectomized rats leads to a prolonged plasma half-life for [125I]β-glucuronidase, a ligand for the mannose receptor. Clearance of [125I]asialofetuin, a ligand for the galactose receptor of hepatocytes, was unaffected by injection of A chain. Microscopic examination of the livers of A chain-treated animals revealed a loss of phagocytic cells from the liver sinusoids. These results suggest that ricin A chain may be useful as a toxin specific for mannose receptor bearing cells of the reticuloendothelial system.

Original languageEnglish
Pages (from-to)849-854
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume146
Issue number2
DOIs
StatePublished - Jul 31 1987

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