In vivo and in vitro assessment of brain bioenergetics in aging rats

Ol'ga Vančová, Ladislav Bačiak, Svatava Kašparová, Jarmila Kucharská, Hector H. Palacios, Jaromír Horeckỳ, Gjumrakch Aliev

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Brain energy disorders can be present in aged men and animals. To this respect, the mitochondrial and free radical theory of aging postulates that age-associated brain energy disorders are caused by an imbalance between pro- and anti-oxidants that can result in oxidative stress. Our study was designed to investigate brain energy metabolism and the activity of endogenous antioxidants during their lifespan in male Wistar rats. In vivo brain bioenergetics were measured using 31P nuclear magnetic resonance (NMR) spectroscopy and in vitro by polarographic analysis of mitochondrial oxidative phosphorylation. When compared to the young controls, a significant decrease of age-dependent mitochondrial respiration and adenosine-3-phosphate (ATP) production measured in vitro correlated with significant reduction of forward creatine kinase reaction (kfor) and with an increase in phosphocreatine (PCr)/ATP, PCr/Pi and PME/ATP ratio measured in vivo. The levels of enzymatic antioxidants catalase, GPx and GST significantly decreased in the brain tissue as well as in the peripheral blood of aged rats. We suppose that mitochondrial dysfunction and oxidative inactivation of endogenous enzymes may participate in age-related disorders of brain energy metabolism.

Original languageEnglish
Pages (from-to)2667-2674
Number of pages8
JournalJournal of Cellular and Molecular Medicine
Volume14
Issue number11
DOIs
StatePublished - Nov 2010

Keywords

  • P NMR
  • Aging
  • Antioxidants
  • Brain
  • Mitochondria
  • OXPHOS

Fingerprint

Dive into the research topics of 'In vivo and in vitro assessment of brain bioenergetics in aging rats'. Together they form a unique fingerprint.

Cite this