In vivo 5-fluorouracil and fluoronucleotide T1 relaxation time measurements using the variable nutation angle method

Terence P.F. Gade, William M. Spees, H. Carl Le, Kristen L. Zakian, Vladimir Ponomarev, Michael Doubrovin, Juri G. Gelovani, Jason A. Koutcher

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8 Scopus citations

Abstract

19Fluorine NMRS has the potential to enable noninvasive predictions of tumor response to 5-fluorouracil (5FU) therapy based on tumor pharmacokinetics. Knowledge of the T1's of 5FU and its fluoronucleotide anabolites (FNuc) is required for quantitative spectral analysis and selection of optimal pulse parameters. We used the variable nutation angle (VNA) method to determine T1's of 5FU and FNuc in subcutaneous Walker 256 rat mammary carcinosarcoma tumors transfected with a cytosine deaminase/uracil phosphoribosyltransferase fusion gene. We calibrated in vivo NAs using methoxydifluoroacetate to ensure the accuracy of these measurements. The T1's were calculated based on signal intensities acquired with NAs of 20°, 35°, 45°, 60°, and 75°. The acquisition order of these NAs was shuffled to reduce the effect of signal variations. The determined T1's for 5FU and FNuc (2.3 ± 0.1 s and 1.3 ± 0.1 s, respectively) represent the first reported in vivo measurements for these metabolites in tumor.

Original languageEnglish
Pages (from-to)169-173
Number of pages5
JournalMagnetic resonance in medicine
Volume52
Issue number1
DOIs
StatePublished - Jul 1 2004

Keywords

  • 5-fluorouracil
  • Fluoronucleotides
  • In vivo
  • T relaxation time
  • Variable nutation angle

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    Gade, T. P. F., Spees, W. M., Le, H. C., Zakian, K. L., Ponomarev, V., Doubrovin, M., Gelovani, J. G., & Koutcher, J. A. (2004). In vivo 5-fluorouracil and fluoronucleotide T1 relaxation time measurements using the variable nutation angle method. Magnetic resonance in medicine, 52(1), 169-173. https://doi.org/10.1002/mrm.20131