In vitro translation of intestinal sucrase-isomaltase and glucoamylase

D. H. Alpers; D. Helms; S. Seetharam; V. L. May; A. W. Strauss

doi:10.1016/0006-291X(86)90523-1

In vitro translation of intestinal sucrase-isomaltase and glucoamylase

D. H. Alpers, D. Helms, S. Seetharam, V. L. May, A. W. Strauss

Gastroenterology

Research output: Contribution to journal › Article

12 Scopus citations

Abstract

It is has been proposed that both sucrase-isomaltase and glucoamylase are initially synthesized as large single-chain precursors which are then processed to heterodimers. We have tested this hypothesis by in vitro translation of their mRNAs. The primary translation product of sucrase-isomaltase mRNA was a single polypeptide of M_r = 190,000. Similar experiments using antiserum against glucoamylase revealed a single polypeptide of M_r = 145,000. These results are consistent with the single chain precursor hypothesis for sucrase-isomaltase. However, the glucoamylase product (145 kDa) is too small to be processed to a heterodimer of M_r = 230,000. Moreover, the mature subunits (M_r = 135,000 and 125,000) probably are derived from the 145 kDa precursor by proteolytic trimming and must include and share most of the precursor protein.

Original language	English
Pages (from-to)	37-43
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	134
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(86)90523-1
State	Published - Jan 14 1986

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Alpers, D. H., Helms, D., Seetharam, S., May, V. L., & Strauss, A. W. (1986). In vitro translation of intestinal sucrase-isomaltase and glucoamylase. Biochemical and Biophysical Research Communications, 134(1), 37-43. https://doi.org/10.1016/0006-291X(86)90523-1

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