In vitro T cell proliferation from kidney allograft biopsies with unremarkable pathology: New strategies for an old problem

Craig R. Smith, Andrés Jaramillo, Nancy J. Poindexter, Nancy S. Steward, Kim C. Lu, Daniel C. Brennan, Gary G. Singer, Brent W. Miller, Martin D. Jendrisak, Surendra Shenoy, Jeffrey A. Lowell, Todd K. Howard, T. Mohanakumar

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Abstract

Acute rejection of renal allografts is mediated by infiltrating alloreactive T cells. The goals of this study were to correlate T cell proliferation with rejection and to determine whether T cell proliferation in the absence of rejection would predict future rejection episodes. Toward this, kidney biopsies (n=100) were cultured in the presence of interleukin-2. Cultures were examined at 4, 24, and 48 hr for T cell proliferation. A strong correlation was observed between T cell proliferation at any time point and rejection. There was not a significant correlation between T cell proliferation in biopsies with no rejection and the occurrence of a rejection episode within 2 months. However, T cell proliferation after 4 hr was a better predictor of the occurrence of rejection within 2 months compared with observations after 24 and 48 hr. Therefore, a subgroup of patients with unremarkable biopsies but T cell proliferation may be at risk for rejection and warrant closer observation and possible tailoring of immunosuppression.

Original languageEnglish
Pages (from-to)142-145
Number of pages4
JournalTransplantation
Volume73
Issue number1
DOIs
StatePublished - Jan 15 2002

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    Smith, C. R., Jaramillo, A., Poindexter, N. J., Steward, N. S., Lu, K. C., Brennan, D. C., Singer, G. G., Miller, B. W., Jendrisak, M. D., Shenoy, S., Lowell, J. A., Howard, T. K., & Mohanakumar, T. (2002). In vitro T cell proliferation from kidney allograft biopsies with unremarkable pathology: New strategies for an old problem. Transplantation, 73(1), 142-145. https://doi.org/10.1097/00007890-200201150-00026