In vitro expression of the alpha and beta subunits of the Na,K-ATPase.

M. Gilmore-Hebert, R. W. Mercer, J. W. Schneider, E. J. Benz

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Our analysis of the cloned alpha 3 protein strongly suggests that this c-DNA represents a bona fide Na+,K+ ATPase isoform. Its similarity to alpha + may have made its detection in tissues by gel migration or immunoreactivity difficult. Expression of an enzymatically active alpha 3 beta Na+K+ATPase either in a completely in vitro system or in a heterologous tissue culture system will clearly establish the biochemical properties of this isoform. Development of alpha 3 specific immunochemical probes will allow a proper assessment of its in vivo expression.

Original languageEnglish
Pages (from-to)71-76
Number of pages6
JournalProgress in Clinical and Biological Research
Volume268 B
StatePublished - 1988
Externally publishedYes

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