In vitro complement activation by ragweed allergen extract in the sera of ragweed allergic and non-allergic persons

Tünde Hidvégi, Béla Schmidt, Lilian Varga, Mária Dervaderics, Ákos Lantos, Zsuzsanna Gönczi, József Barok, Miklós Otos, Michael Kirschfink, Peter Späth, George Füst

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19 Scopus citations

Abstract

Ragweed allergen (RWA)-induced complement activation in sera of 40 RW allergic patients and of 40 non-allergic controls was investigated. After treatment of the sera with RWA, levels of C3a, C5a, C3bBbP, ClrClsClinh, SC5b-9 and granulocyte-aggregating activity were determined. Concentration of RW-specific IgG was also measured. After RWA treatment dose-dependent complement activation was detected in sera of RW allergic and non-allergic persons. C3a generation was observed mostly in the sera of RW allergic individuals, while levels of C3bBbP and of RW-specific IgG were significantly higher in sera of allergics, and a strong correlation was found between these two parameters. In a prospective clinical study, a significant positive correlation was observed between the extent of RWA-induced alternative pathway (AP) activation and the severity of symptoms of allergic rhinoconjunctivitis that were developed in a 4-week period subsequent to blood sampling. These observations suggest that complement activation has a role in the development of the symptoms of RW allergy.

Original languageEnglish
Pages (from-to)65-71
Number of pages7
JournalImmunology Letters
Volume48
Issue number1
DOIs
StatePublished - Nov 1995

Keywords

  • Asthma bronchiale
  • Complement activation
  • Ragweed allergy
  • Rhinoconjunctivitis

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