In vitro calcite crystal morphology is modulated by otoconial proteins Otolin-1 and Otoconin-90

K. Trent Moreland, Mina Hong, Wenfu Lu, Christopher W. Rowley, David M. Ornitz, James J. De Yoreo, Ruediger Thalmann

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Otoconia are formed embryonically and are instrumental in detecting linear acceleration and gravity. Degeneration and fragmentation of otoconia in elderly patients leads to imbalance resulting in higher frequency of falls that are positively correlated with the incidence of bone fractures and death. In this work we investigate the roles otoconial proteins Otolin-1 and Otoconin 90 (OC90) perform in the formation of otoconia. We demonstrate by rotary shadowing and atomic force microscopy (AFM) experiments that Otolin-1 forms homomeric protein complexes and self-assembled networks supporting the hypothesis that Otolin-1 serves as a scaffold protein of otoconia. Our calcium carbonate crystal growth data demonstrate that Otolin-1 and OC90 modulate in vitro calcite crystal morphology but neither protein is sufficient to produce the shape of otoconia. Coadministration of these proteins produces synergistic effects on crystal morphology that contribute to morphology resembling otoconia.

Original languageEnglish
Article numbere95333
JournalPloS one
Volume9
Issue number4
DOIs
StatePublished - Apr 18 2014

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