In Vitro and In Vivo Gene Delivery Mediated By A Synthetic Polycationic Amino Polymer

Corey K. Goldman, Lilians Soroceanu, Natalie Smith, G. Yancey Gillespie, Walter Shaw, Steve Burgess, Guadalupe Bilbao, David T. Curiel

Research output: Contribution to journalArticle

100 Scopus citations

Abstract

A synthetic polyamino polymer with a glucose backbone was used for gene transfer in vitro and in vivo. Gene transfer in vitro to various human carcinoma cell lines was achieved with an efficiency superior to a commercially available cationic liposome preparation. The polymer was resistant to inhibition by serum, which allowed for efficient gene transfer in vivo. Direct intracranial tumor injection using this reagent resulted in reporter gene expression levels comparable to those achieved by a recombinant adenoviral vector. Thus, this compound represents a new class of agent that may have broad utility for gene transfer and gene therapy applications.

Original languageEnglish
Pages (from-to)462-466
Number of pages5
JournalNature Biotechnology
Volume15
Issue number5
DOIs
StatePublished - May 1997
Externally publishedYes

Keywords

  • Gene therapy
  • Gene transfer
  • Liposome
  • Transfection

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    Goldman, C. K., Soroceanu, L., Smith, N., Gillespie, G. Y., Shaw, W., Burgess, S., Bilbao, G., & Curiel, D. T. (1997). In Vitro and In Vivo Gene Delivery Mediated By A Synthetic Polycationic Amino Polymer. Nature Biotechnology, 15(5), 462-466. https://doi.org/10.1038/nbt0597-462