TY - JOUR
T1 - In situ surface-enhanced Raman spectroelectrochemical analysis system with a hemin modified nanostructured gold surface
AU - Yuan, Tao
AU - Le Thi Ngoc, Loan
AU - Van Nieuwkasteele, Jan
AU - Odijk, Mathieu
AU - Van Den Berg, Albert
AU - Permentier, Hjalmar
AU - Bischoff, Rainer
AU - Carlen, Edwin T.
N1 - Publisher Copyright:
© 2015 American Chemical Society.
PY - 2015/3/3
Y1 - 2015/3/3
N2 - An integrated surface-enhanced Raman scattering (SERS) spectroelectrochemical (SEC) analysis system is presented that combines a small volume microfluidic sample chamber (<100 μL) with a compact three-electrode configuration for in situ surface-enhanced Raman spectroelectrochemistry. The SEC system includes a nanostructured Au surface that serves dual roles as the electrochemical working electrode (WE) and SERS substrate, a microfabricated Pt counter electrode (CE), and an external Ag/AgCl reference electrode (RE). The nanostructured Au WE enables highly sensitive in situ SERS spectroscopy through large and reproducible SERS enhancements, which eliminates the need for resonant wavelength matching of the laser excitation source with the electronic absorption of the target molecule. The new SEC analysis system has the merits of wide applicability to target molecules, small sample volume, and a low detection limit. We demonstrate in situ SERS spectroelectrochemistry measurements of the metalloporphyrin hemin showing shifts of the iron oxidation marker band ν4 with the nanostructured Au working electrode under precise potential control.
AB - An integrated surface-enhanced Raman scattering (SERS) spectroelectrochemical (SEC) analysis system is presented that combines a small volume microfluidic sample chamber (<100 μL) with a compact three-electrode configuration for in situ surface-enhanced Raman spectroelectrochemistry. The SEC system includes a nanostructured Au surface that serves dual roles as the electrochemical working electrode (WE) and SERS substrate, a microfabricated Pt counter electrode (CE), and an external Ag/AgCl reference electrode (RE). The nanostructured Au WE enables highly sensitive in situ SERS spectroscopy through large and reproducible SERS enhancements, which eliminates the need for resonant wavelength matching of the laser excitation source with the electronic absorption of the target molecule. The new SEC analysis system has the merits of wide applicability to target molecules, small sample volume, and a low detection limit. We demonstrate in situ SERS spectroelectrochemistry measurements of the metalloporphyrin hemin showing shifts of the iron oxidation marker band ν4 with the nanostructured Au working electrode under precise potential control.
UR - https://www.scopus.com/pages/publications/84923859061
U2 - 10.1021/ac504136j
DO - 10.1021/ac504136j
M3 - Article
C2 - 25643066
AN - SCOPUS:84923859061
SN - 0003-2700
VL - 87
SP - 2588
EP - 2592
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 5
ER -