In situ structure and organization of the influenza C virus surface glycoprotein

Steinar Halldorsson, Kasim Sader, Jack Turner, Lesley J. Calder, Peter B. Rosenthal

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


The lipid-enveloped influenza C virus contains a single surface glycoprotein, the haemagglutinin-esterase-fusion (HEF) protein, that mediates receptor binding, receptor destruction, and membrane fusion at the low pH of the endosome. Here we apply electron cryotomography and subtomogram averaging to describe the structural basis for hexagonal lattice formation by HEF on the viral surface. The conformation of the glycoprotein in situ is distinct from the structure of the isolated trimeric ectodomain, showing that a splaying of the membrane distal domains is required to mediate contacts that form the lattice. The splaying of these domains is also coupled to changes in the structure of the stem region which is involved in membrane fusion, thereby linking HEF’s membrane fusion conformation with its assembly on the virus surface. The glycoprotein lattice can form independent of other virion components but we show a major role for the matrix layer in particle formation.

Original languageEnglish
Article number1694
JournalNature communications
Issue number1
StatePublished - Dec 1 2021


Dive into the research topics of 'In situ structure and organization of the influenza C virus surface glycoprotein'. Together they form a unique fingerprint.

Cite this