In situ friction measurement on murine cartilage by atomic force microscopy

Jeffrey M. Coles, Jason J. Blum, Gregory D. Jay, Eric M. Darling, Farshid Guilak, Stefan Zauscher

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

Articular cartilage provides a low-friction, wear-resistant surface for the motion of diarthrodial joints. The objective of this study was to develop a method for in situ friction measurement of murine cartilage using a colloidal probe attached to the cantilever of an atomic force microscope. Sliding friction was measured between a chemically functionalized microsphere and the cartilage of the murine femoral head. Friction was measured at normal loads ranging incrementally from 20 to 100 nN with a sliding speed of 40 μm/s and sliding distance of 64 μm. Under these test conditions, hydrostatic pressurization and biphasic load support in the cartilage were minimized, providing frictional measurements that predominantly reflect boundary lubrication properties. Friction coefficients measured on murine tissue (0.25±0.11) were similar to those measured on porcine tissue (0.23±0.09) and were in general agreement with measurements of boundary friction on cartilage by other researchers. Using the colloidal probe as an indenter, the elastic mechanical properties and surface roughness were measured in the same configuration. Interfacial shear was found to be the principal mechanism of friction generation, with little to no friction resulting from plowing forces, collision forces, or energy losses due to normal deformation. This measurement technique can be applied to future studies of cartilage friction and mechanical properties on genetically altered mice or other small animals.

Original languageEnglish
Pages (from-to)541-548
Number of pages8
JournalJournal of Biomechanics
Volume41
Issue number3
DOIs
StatePublished - Feb 5 2008
Externally publishedYes

Keywords

  • Boundary lubrication
  • Lubricin
  • Scanning probe microscopy
  • Tribology

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