Improving the Sensitivity of FAB Tandem Mass Spectrometry for PAH-DNA Adducts.

Joelle Wellemans; Mathai George; Ronald L. Cerny; Michael L. Gross

doi:10.1080/10406639408031173

Improving the Sensitivity of FAB Tandem Mass Spectrometry for PAH-DNA Adducts.

Joelle Wellemans, Mathai George, Ronald L. Cerny, Michael L. Gross

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The mass spectrometric detection limits for guanine, adenine, and an adenine/dimethylbenzanthracene adduct were greatly improved by converting the materials to pentafluorobenzyl derivatives, as well as by doing coaxial continuous flow-FAB (coaxial cf-Fast Atom Bombardment). The lowest detection limit (approximately 10 pg) was achieved when the derivatized sample was introduced by coaxial cf-FAB and the determination was by full-scan mass spectrometry. The lowest detection limit achieved by tandem mass spectrometry was 50 pg obtained after derivatization, coaxial cf-FAB introduction and detection with an extended mass range array.

Original language	English
Pages (from-to)	103-110
Number of pages	8
Journal	Polycyclic Aromatic Compounds
Volume	6
Issue number	1-4
DOIs	https://doi.org/10.1080/10406639408031173
State	Published - Jan 1994

Keywords

array detector
coaxial cf-FAB
derivatization
detection limit
PAH-DNA adducts
Tandem MS

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10.1080/10406639408031173

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title = "Improving the Sensitivity of FAB Tandem Mass Spectrometry for PAH-DNA Adducts.",

abstract = "The mass spectrometric detection limits for guanine, adenine, and an adenine/dimethylbenzanthracene adduct were greatly improved by converting the materials to pentafluorobenzyl derivatives, as well as by doing coaxial continuous flow-FAB (coaxial cf-Fast Atom Bombardment). The lowest detection limit (approximately 10 pg) was achieved when the derivatized sample was introduced by coaxial cf-FAB and the determination was by full-scan mass spectrometry. The lowest detection limit achieved by tandem mass spectrometry was 50 pg obtained after derivatization, coaxial cf-FAB introduction and detection with an extended mass range array.",

keywords = "array detector, coaxial cf-FAB, derivatization, detection limit, PAH-DNA adducts, Tandem MS",

author = "Joelle Wellemans and Mathai George and Cerny, {Ronald L.} and Gross, {Michael L.}",

note = "Funding Information: ACKNOWLEDGEMENTS This work was supported by U.S. National Institutes of Health (Grant No. 1P O 1C A492 10-05).",

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AU - Wellemans, Joelle

AU - George, Mathai

AU - Cerny, Ronald L.

AU - Gross, Michael L.

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N2 - The mass spectrometric detection limits for guanine, adenine, and an adenine/dimethylbenzanthracene adduct were greatly improved by converting the materials to pentafluorobenzyl derivatives, as well as by doing coaxial continuous flow-FAB (coaxial cf-Fast Atom Bombardment). The lowest detection limit (approximately 10 pg) was achieved when the derivatized sample was introduced by coaxial cf-FAB and the determination was by full-scan mass spectrometry. The lowest detection limit achieved by tandem mass spectrometry was 50 pg obtained after derivatization, coaxial cf-FAB introduction and detection with an extended mass range array.

AB - The mass spectrometric detection limits for guanine, adenine, and an adenine/dimethylbenzanthracene adduct were greatly improved by converting the materials to pentafluorobenzyl derivatives, as well as by doing coaxial continuous flow-FAB (coaxial cf-Fast Atom Bombardment). The lowest detection limit (approximately 10 pg) was achieved when the derivatized sample was introduced by coaxial cf-FAB and the determination was by full-scan mass spectrometry. The lowest detection limit achieved by tandem mass spectrometry was 50 pg obtained after derivatization, coaxial cf-FAB introduction and detection with an extended mass range array.

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KW - coaxial cf-FAB

KW - derivatization

KW - detection limit

KW - PAH-DNA adducts

KW - Tandem MS

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Improving the Sensitivity of FAB Tandem Mass Spectrometry for PAH-DNA Adducts.

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Keywords

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