Improvement in the apparent mass resolution of oligonucleotides by using 12C/14N-enriched samples

K. Tang; M. Shahgholi; B. A. Garcia; P. J. Heaney; C. R. Cantor; L. G. Scott; J. R. Williamson

doi:10.1021/ac010804c

Improvement in the apparent mass resolution of oligonucleotides by using ¹²C/¹⁴N-enriched samples

K. Tang, M. Shahgholi, B. A. Garcia, P. J. Heaney, C. R. Cantor, L. G. Scott, J. R. Williamson

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8 Scopus citations

Abstract

The apparent mass resolution of oligonucleotides in time-of-flight (TOF) mass spectrometers has been examined. In a reflectron TOF instrument, where the isotopic profile can be completely resolved, the apparent resolution matches the instrument's resolving power. In a linear TOF instrument, unresolved isotopic profiles limit the apparent resolution to much lower values than the actual instrument resolution. By using ¹²C/¹⁴N-enriched oligonucleotides, the apparent resolution can be improved significantly. The isotope enrichment method also enhances the signal-to-noise ratio.

Original language	English
Pages (from-to)	226-231
Number of pages	6
Journal	Analytical Chemistry
Volume	74
Issue number	1
DOIs	https://doi.org/10.1021/ac010804c
State	Published - Jan 1 2002

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abstract = "The apparent mass resolution of oligonucleotides in time-of-flight (TOF) mass spectrometers has been examined. In a reflectron TOF instrument, where the isotopic profile can be completely resolved, the apparent resolution matches the instrument's resolving power. In a linear TOF instrument, unresolved isotopic profiles limit the apparent resolution to much lower values than the actual instrument resolution. By using 12C/14N-enriched oligonucleotides, the apparent resolution can be improved significantly. The isotope enrichment method also enhances the signal-to-noise ratio.",

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AU - Tang, K.

AU - Shahgholi, M.

AU - Garcia, B. A.

AU - Heaney, P. J.

AU - Cantor, C. R.

AU - Scott, L. G.

AU - Williamson, J. R.

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Y1 - 2002/1/1

N2 - The apparent mass resolution of oligonucleotides in time-of-flight (TOF) mass spectrometers has been examined. In a reflectron TOF instrument, where the isotopic profile can be completely resolved, the apparent resolution matches the instrument's resolving power. In a linear TOF instrument, unresolved isotopic profiles limit the apparent resolution to much lower values than the actual instrument resolution. By using 12C/14N-enriched oligonucleotides, the apparent resolution can be improved significantly. The isotope enrichment method also enhances the signal-to-noise ratio.

AB - The apparent mass resolution of oligonucleotides in time-of-flight (TOF) mass spectrometers has been examined. In a reflectron TOF instrument, where the isotopic profile can be completely resolved, the apparent resolution matches the instrument's resolving power. In a linear TOF instrument, unresolved isotopic profiles limit the apparent resolution to much lower values than the actual instrument resolution. By using 12C/14N-enriched oligonucleotides, the apparent resolution can be improved significantly. The isotope enrichment method also enhances the signal-to-noise ratio.

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JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 1

ER -

Improvement in the apparent mass resolution of oligonucleotides by using ¹²C/¹⁴N-enriched samples

Abstract

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