Improved transmission electron microscopy: Technique for the study of cytologic material

S. Hultgren, D. F. Hidvegi

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

A modification of the technique of Coleman et al for the preparation of single cells in cytologic specimens for electron microscopy (EM) is described. By employing materials in the initial cytologic processing that are useful for EM, such as a paraformaldehyde glutaraldehyde fixative, lactated Ringer's solution as a rinsing medium, glycerol as a mounting medium and cacodylate buffer for removal of coverslips, the use of alcohol fixatives and standard mounting media could be avoided. This preserved the cytoplasmic detail, which is usually degenerated in cells removed from cytologic specimens and processed for EM.

Original languageEnglish
Pages (from-to)179-183
Number of pages5
JournalActa Cytologica
Volume29
Issue number2
StatePublished - Jan 1 1985
Externally publishedYes

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