Improved targeting of ligand-modified adenovirus as a new near infrared fluorescence tumor imaging probe

Lingling Shan, Jianpeng Xue, Jing Guo, Zhiyu Qian, Samuel Achilefu, Yueqing Gu

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

E1/E3-deleted Adenovirus 5 (Ad.5) possesses a great potential in gene therapy because of its high efficacy in gene transfer and low toxicity. Studies have shown that Coxsackie-Adenovirus receptor (CAR) is the determinant factor for the targeting of Adenovirus vectors. To extend the natural targeting of Ad to low CAR expressing tumors, we covalently attached folic acid (FA) to E1/E3-deleted Ad.5 capsids. Near-infrared (NIR) fluorescent dye ICG-Der-02 was subsequently conjugated with FA-Ad particles for in vivo imaging. The cell experiments and acute toxicity studies demonstrated the low toxicity of FA-Ad-ICG02 to normal cell/tissues. The dynamic behavior and targeting ability of FA-Ad-ICG02 to different tumors were investigated by NIR fluorescence imaging. In vitro and in vivo studies demonstrated its high targeting capability to CAR or FR positive tumors. The results support the potential of using ligand-modified Ad probe for tumor diagnosis and targeted therapy.

Original languageEnglish
Pages (from-to)567-581
Number of pages15
JournalBioconjugate Chemistry
Volume22
Issue number4
DOIs
StatePublished - Apr 20 2011

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