Improved monoclonal antibody tumor/background ratios with exchange transfusions

Christine A. Henry, Anaira C. Clavo, Richard L. Wahl

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Blood exchange transfusions were performed in nude rats with subcutaneous HTB77 human ovarian carcinoma xenografts in an attempt to improve specific monoclonal antibody (MoAb) tumor/non-tumor uptake ratios. Animals were injected intravenously with both 131I-5G6.4 specific and 125I-UPC-10 non-specific MoAb. Twenty-four hours later 65-80% of the original blood was exchanged with normal heparinized rat blood and then these rodents were sacrificed. Exchange transfusion significantly (P < 0.05) decreased normal tissue activities of 131I (except for muscle) by 63-85%. while tumor activity decreased only 5%. Tumor to background ratios increased from 0.1-0.8 to 2.3-6.3. Exchange transfusions substantially enhance tumor/normal tissue antibody uptake ratios and, along with plasmapheresis, may be useful in enhancing antibody localization in vivo, particularly for therapy.

Original languageEnglish
Pages (from-to)565-567
Number of pages3
JournalInternational Journal of Radiation Applications and Instrumentation.
Volume18
Issue number5
DOIs
StatePublished - 1991

Fingerprint Dive into the research topics of 'Improved monoclonal antibody tumor/background ratios with exchange transfusions'. Together they form a unique fingerprint.

  • Cite this