TY - JOUR
T1 - Improved microradiographic contrast for bone stain-historadiography
AU - Simmons, David J.
AU - Bates, Margaret
AU - Teitelbaum, Steven L.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1976
Y1 - 1976
N2 - Microradiographs of 5-micron sections of methyl methacrylate embedded undemineralized bone show poor resolution, but prestaining with silver nitrate increases the radioopacity of the calcified tissues to soft x-rays without masking regional differences in microscopic mineralization. Identical results are achieved using aqueous (pH 5.8 and 7.5) or ammoniacal solutions (pH 7.9). Atomic absorption spectrometry detected no loss of calcium from the sections during staining. Osteoid in plastic-embedded bone is not rendered radiopaque by this technique even after prolonged staining times (5 min-2 hr).
AB - Microradiographs of 5-micron sections of methyl methacrylate embedded undemineralized bone show poor resolution, but prestaining with silver nitrate increases the radioopacity of the calcified tissues to soft x-rays without masking regional differences in microscopic mineralization. Identical results are achieved using aqueous (pH 5.8 and 7.5) or ammoniacal solutions (pH 7.9). Atomic absorption spectrometry detected no loss of calcium from the sections during staining. Osteoid in plastic-embedded bone is not rendered radiopaque by this technique even after prolonged staining times (5 min-2 hr).
UR - http://www.scopus.com/inward/record.url?scp=84907130393&partnerID=8YFLogxK
U2 - 10.3109/10520297609116691
DO - 10.3109/10520297609116691
M3 - Article
C2 - 59423
AN - SCOPUS:84907130393
SN - 1052-0295
VL - 51
SP - 153
EP - 157
JO - Biotechnic and Histochemistry
JF - Biotechnic and Histochemistry
IS - 3
ER -