Improved determination of hydrogen peroxide or lucigenin by measurement of lucigenin chemiluminescence in organised assemblies

Cheryl L. Malehorn, Terrence E. Riehl, Willie L. Hinze

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Chemiluminescence (CL) from the reaction of 10,10′-dimethyl-9, 9′-biacridinium dinitrate (lucigenin) with hydrogen peroxide in the presence of different organised assemblies has been evaluated as a means of improving the CL assay for the determination of hydrogen peroxide. If the assay is conducted in the presence of 3-(N-dodecyl-N,N-dimethylammonio)propane-1- sulphonate, hexadecyltrimethylammonium chloride or polyoxyethylene(23)dodecanol (Brij-35) micelles or β-cyclodextrin, improvements in the sensitivity by factors of 1.9-7.6 were observed relative to that in water. The best sensitivity is achieved in micellar Brij-35 in which the CL intensity is linearly proportional to hydrogen peroxide from 3.0 × 10-7 up to 1.0 × 10-3 M (the highest concentration examined). The precision in Brij-35 is good with the relative deviation at the 95% confidence level being 3.2 × 2.2%. The selectivity of the lucigenin CL assay for hydrogen peroxide is also improved in the Brij-35 medium. Additionally, the usable pH range in which analytically useful CL can be observed is extended in the Brij-35 or CTAC micellar systems relative to water. Preliminary data suggest that these advantages are also possible if one uses the reaction to quantitate lucigenin rather than hydrogen peroxide.

Original languageEnglish
Pages (from-to)941-947
Number of pages7
JournalThe Analyst
Volume111
Issue number8
DOIs
StatePublished - Jan 1 1986

Keywords

  • Brij-35
  • Cyclodextrins
  • Hydrogen peroxide determination
  • Lucigenin chemiluminescence
  • Lucigenin determination

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