TY - JOUR
T1 - Improved detection sensitivity of line-scanning optical coherence microscopy
AU - Chen, Yu
AU - Huang, Shu Wei
AU - Zhou, Chao
AU - Potsaid, Benjamin
AU - Fujimoto, James G.
N1 - Funding Information:
Manuscript received May 16, 2011; revised June 21, 2011; accepted July 5, 2011. Date of publication July 14, 2011; date of current version June 1, 2012. The work of Y. Chen was supported by the Nano-Biotechnology Award of State of Maryland, the Prevent Cancer Foundation, the UMB-UMCP Seed Grant Program, the A. Ward Ford Memorial Institute Fund, and the National Institutes of Health R21-AR059325-01 and R21-EB012215-01A1. The work of J. G. Fu-jimoto was supported by the National Institutes of Health R01-CA75289-14, R01-EY11289-25, R01-HL095717-01, and R01-NS057476-02; the Air Force Office of Scientific Research FA9550-10-1-0551 and FA9550-10-1-0063. The work of C. Zhou was supported by the National Institutes of Health K99-EB010071-01A1. Y. Chen and S.-W. Huang contributed equally to this work.
PY - 2012
Y1 - 2012
N2 - Optical coherence microscopy (OCM) is a promising technology for high-resolution cellular-level imaging in human tissues. Line-scanning OCM is a new form of OCM that utilizes line-field illumination for parallel detection. In this study, we demonstrate improved detection sensitivity by using an achromatic design for line-field generation. This system operates at 830-nm wavelength with 82-nm bandwidth. The measured axial resolution is 3.9 μm in air (corresponding to ∼2.9 μm in tissue), and the transverse resolutions are 2.1 μm along the line-field illumination direction and 1.7 μm perpendicular to line illumination direction. The measured sensitivity is 98 dB with 25 line averages, resulting in an imaging speed of ∼2 frames/s (516 lines/s). Real-time, cellular-level imaging of scattering tissues is demonstrated using human-colon specimens.
AB - Optical coherence microscopy (OCM) is a promising technology for high-resolution cellular-level imaging in human tissues. Line-scanning OCM is a new form of OCM that utilizes line-field illumination for parallel detection. In this study, we demonstrate improved detection sensitivity by using an achromatic design for line-field generation. This system operates at 830-nm wavelength with 82-nm bandwidth. The measured axial resolution is 3.9 μm in air (corresponding to ∼2.9 μm in tissue), and the transverse resolutions are 2.1 μm along the line-field illumination direction and 1.7 μm perpendicular to line illumination direction. The measured sensitivity is 98 dB with 25 line averages, resulting in an imaging speed of ∼2 frames/s (516 lines/s). Real-time, cellular-level imaging of scattering tissues is demonstrated using human-colon specimens.
KW - Confocal microscopy
KW - medical and biological imaging
KW - optical coherence microscopy (OCM)
KW - optical coherence tomography (OCT)
UR - http://www.scopus.com/inward/record.url?scp=84862300531&partnerID=8YFLogxK
U2 - 10.1109/JSTQE.2011.2161758
DO - 10.1109/JSTQE.2011.2161758
M3 - Article
AN - SCOPUS:84862300531
SN - 1077-260X
VL - 18
SP - 1094
EP - 1099
JO - IEEE Journal on Selected Topics in Quantum Electronics
JF - IEEE Journal on Selected Topics in Quantum Electronics
IS - 3
M1 - 5951723
ER -