TY - JOUR
T1 - Improved cell surface radioiodination of macrophages
AU - Kulczycki, Anthony
AU - Krause, Valerie
AU - Killion, Catherine Chew
AU - Atkinson, John P.
N1 - Funding Information:
The assistance of Ms. Mary Anne Blue and Ms. Lorraine Bourisaw in the preparation of this manuscript is greatly appreciated. This work was supported in part by the Centers for Interdisciplinary Research in Immunological Diseases (CIRID) Grant 1 P50 AI 15322 and NIH Grant 1 R01-AI 14494-02.
PY - 1980
Y1 - 1980
N2 - Rabbit alveolar macrophages at high cell concentrations are inefficiently radiolabeled by the usual lactoperoxidase-catalyzed iodination method. Soluble macromolecules secreted by macrophages were found to inhibit the radioiodination of macrophages and other cells. A modified procedure is described which minimizes the presence of such inihibitory material and thereby considerably improves the radioiodination efficiency for both alviolar macrophages and the macrophage-like P388D1 cell line.
AB - Rabbit alveolar macrophages at high cell concentrations are inefficiently radiolabeled by the usual lactoperoxidase-catalyzed iodination method. Soluble macromolecules secreted by macrophages were found to inhibit the radioiodination of macrophages and other cells. A modified procedure is described which minimizes the presence of such inihibitory material and thereby considerably improves the radioiodination efficiency for both alviolar macrophages and the macrophage-like P388D1 cell line.
UR - http://www.scopus.com/inward/record.url?scp=0018952184&partnerID=8YFLogxK
U2 - 10.1016/0022-1759(80)90198-2
DO - 10.1016/0022-1759(80)90198-2
M3 - Article
C2 - 7003011
AN - SCOPUS:0018952184
SN - 0022-1759
VL - 37
SP - 133
EP - 138
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 2
ER -