Impairment of V(D)J recombination in double-strand break repair mutants

Guillermo E. Taccioli, Gary Rathbun, Eugene Oltz, Thomas Stamato, Penny A. Jeggo, Frederick W. Alt

Research output: Contribution to journalArticle

410 Scopus citations

Abstract

Cells maintain the integrity of their genome through an intricate network of repair systems that recognize and remove lesions from DNA. The only known site-directed recombination process in vertebrates is the V(D)J recombination of lymphocyte antigen receptor genes. A large panel of cell lines deficient in DNA repair were tested for the ability to perform V(D)J recombination after introduction of the RAG-1 and RAG-2 genes. Two mutants failed to generate normal V(D)J recombination, and further analysis provided evidence for two distinct nonlymphoid-specific genes that encode factors involved in both DNA repair and V(D)J recombination.

Original languageEnglish
Pages (from-to)207-210
Number of pages4
JournalScience
Volume260
Issue number5105
DOIs
StatePublished - Jan 1 1993

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    Taccioli, G. E., Rathbun, G., Oltz, E., Stamato, T., Jeggo, P. A., & Alt, F. W. (1993). Impairment of V(D)J recombination in double-strand break repair mutants. Science, 260(5105), 207-210. https://doi.org/10.1126/science.8469973