Antibodies specific for N6 methyladenosine (m6A) and for 7-methylguanosine (m7G) were immobilized on Sepharose and the resulting immunoadsorbents tested for their ability to retain specific oligonucleotides possessing the corresponding antigenic haptens (i.e. m6A and m7G). Results obtained with oligonucleotides derived from ribonuclease T1 digests of Escherichia coli tRNA (previously labeled with [methyl 3H]methionine) indicated that each immunoadsorbent quantitatively and exclusively retained those methyl-3H-labelled oligonucleotides possessing [methyl-3H]m6A and [methyl-3#]m7G. Elution and subsequent characterization of [methyl-3H]m7G. retained methyl-3H labeled oligonucleotides via DEAE-cellulose chromatography revealed the presence of several small oligonucleotides containing m7G and a single, larger oligonucleotide containing m6A. These findings are in accord with previously sequenced structures which indicate that numerous bacterial tRNA species possess m7G while only tRNA(Val) contains m6A.
|Number of pages||3|
|Journal||Journal of Biological Chemistry|
|State||Published - 1977|