Abstract

Immunophenotyping allows for the deep characterization of leukocytes present in biological samples. Here, we describe a complete procedure for the immunophenotyping of amniotic fluid, which can provide information into the immune processes taking place in the amniotic cavity. The protocol describes amniotic fluid cell count determination, processing, and the use of viability, extracellular antibody, and intracellular/intranuclear antibody staining prior to flow cytometer acquisition.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages155-162
Number of pages8
DOIs
StatePublished - 2024

Publication series

NameMethods in Molecular Biology
Volume2781
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Amniotic cavity
  • Flow cytometry
  • Immune cells
  • Pregnancy

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