@article{6dae01c32a9f44fba998fa256b87fae5,
title = "Immunomodulatory lipid mediator profiling of cerebrospinal fluid following surgery in older adults",
abstract = "Arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA) derived lipids play key roles in initiating and resolving inflammation. Neuro-inflammation is thought to play a causal role in perioperative neurocognitive disorders, yet the role of these lipids in the human central nervous system in such disorders is unclear. Here we used liquid chromatography–mass spectrometry to quantify AA, DHA, and EPA derived lipid levels in non-centrifuged cerebrospinal fluid (CSF), centrifuged CSF pellets, and centrifuged CSF supernatants of older adults obtained before, 24 h and 6 weeks after surgery. GAGE analysis was used to determine AA, DHA and EPA metabolite pathway changes over time. Lipid mediators derived from AA, DHA and EPA were detected in all sample types. Postoperative lipid mediator changes were not significant in non-centrifuged CSF (p > 0.05 for all three pathways). The AA metabolite pathway showed significant changes in centrifuged CSF pellets and supernatants from before to 24 h after surgery (p = 0.0000247, p = 0.0155 respectively), from before to 6 weeks after surgery (p = 0.0000497, p = 0.0155, respectively), and from 24 h to 6 weeks after surgery (p = 0.0000499, p = 0.00363, respectively). These findings indicate that AA, DHA, and EPA derived lipids are detectable in human CSF, and the AA metabolite pathway shows postoperative changes in centrifuged CSF pellets and supernatants.",
author = "{for the MADCO-PC Study Team} and Niccol{\`o} Terrando and Park, {John J.} and Michael Devinney and Cliburn Chan and Mary Cooter and Pallavi Avasarala and Mathew, {Joseph P.} and Quinones, {Quintin J.} and Maddipati, {Krishna Rao} and Miles Berger and Brian Brigman and Jeffrey Browndyke and Bullock, {William M.} and Jessica Carter and Joseph Chapman and Brian Colin and D{\textquoteright}Amico, {Thomas A.} and DeOrio, {James K.} and Esclamado, {Ramon M.} and Ferrandino, {Michael N.} and Jeffrey Gadsden and Garrigues, {Grant E.} and Jason Guercio and Ashraf Habib and Harpole, {David H.} and Hartwig, {Mathew G.} and Ehimemen Iboaya and Inman, {Brant A.} and Anver Khan and Sandhya Lagoo-Deenadayalan and Lee, {Paula S.} and Lee, {Walter T.} and John Lemm and Howard Levinson and Christopher Mantyh and McDonagh, {David L.} and John Migaly and Mithani, {Suhail K.} and Eugene Moretti and Moul, {Judd W.} and Newman, {Mark F.} and Brian Ohlendorf and Alexander Perez and Peterson, {Andrew C.} and Preminger, {Glenn M.} and Robertson, {Cary N.} and Roman, {Sanziana A.} and Scott Runyon and Aaron Sandler and Sbahi, {Faris M.} and Scheri, {Randall P.} and Smith, {S. Kendall} and Leonard Talbot and Thacker, {Julie K.M.} and Jake Thomas and Tong, {Betty C.} and Vaslef, {Steven N.} and Nathan Waldron and Xueyuan Wang and Christopher Young",
note = "Funding Information: This work was supported by Duke CTSA grant (UL1TR001117) as well as Duke Anesthesiology departmental funds, and a mentored research grant from the International Anesthesia Research Society. Dr Berger also acknowledges additional support from National Institutes of Health (NIH) grants T32 GM08600, R03AG050918, 1K76AG057022, and the Duke Claude D. Pepper Older American Independence Center (P30AG028716), and a William L. Young neuroscience research award from the Society for Neuroscience in Anesthesiology and Critical Care (SNACC). KM also acknowledges support from the National Center for Research Resources, NIH Grant S10RR027926. Publisher Copyright: {\textcopyright} 2021, The Author(s).",
year = "2021",
month = dec,
doi = "10.1038/s41598-021-82606-5",
language = "English",
volume = "11",
journal = "Scientific reports",
issn = "2045-2322",
number = "1",
}