TY - JOUR
T1 - Immunological Impact of a Gluten-Free Dairy-Free Diet in Children With Kidney Disease
T2 - A Feasibility Study
AU - Pérez-Sáez, María José
AU - Uffing, Audrey
AU - Leon, Juliette
AU - Murakami, Naoka
AU - Watanabe, Andreia
AU - Borges, Thiago J.
AU - Sabbisetti, Venkata S.
AU - Cureton, Pamela
AU - Kenyon, Victoria
AU - Keating, Leigh
AU - Yee, Karen
AU - Fernandes Satiro, Carla Aline
AU - Serena, Gloria
AU - Hildebrandt, Friedhelm
AU - Riella, Cristian V.
AU - Libermann, Towia A.
AU - Wang, Minxian
AU - Pascual, Julio
AU - Bonventre, Joseph V.
AU - Cravedil, Paolo
AU - Fasano, Alessio
AU - Riella, Leonardo V.
N1 - Publisher Copyright:
© Copyright © 2021 Pérez-Sáez, Uffing, Leon, Murakami, Watanabe, Borges, Sabbisetti, Cureton, Kenyon, Keating, Yee, Fernandes Satiro, Serena, Hildebrandt, Riella, Libermann, Wang, Pascual, Bonventre, Cravedil, Fasano and Riella.
PY - 2021/6/2
Y1 - 2021/6/2
N2 - Kidney disease affects 10% of the world population and is associated with increased mortality. Steroid-resistant nephrotic syndrome (SRNS) is a leading cause of end-stage kidney disease in children, often failing standard immunosuppression. Here, we report the results of a prospective study to investigate the immunological impact and safety of a gluten-free and dairy-free (GF/DF) diet in children with SRNS. The study was organized as a four-week summer camp implementing a strict GF/DF diet with prospective collection of blood, urine and stool in addition to whole exome sequencing WES of DNA of participants. Using flow cytometry, proteomic assays and microbiome metagenomics, we show that GF/DF diet had a major anti-inflammatory effect in all participants both at the protein and cellular level with 4-fold increase in T regulatory/T helper 17 cells ratio and the promotion of a favorable regulatory gut microbiota. Overall, GF/DF can have a significant anti-inflammatory effect in children with SRNS and further trials are warranted to investigate this potential dietary intervention in children with SRNS.
AB - Kidney disease affects 10% of the world population and is associated with increased mortality. Steroid-resistant nephrotic syndrome (SRNS) is a leading cause of end-stage kidney disease in children, often failing standard immunosuppression. Here, we report the results of a prospective study to investigate the immunological impact and safety of a gluten-free and dairy-free (GF/DF) diet in children with SRNS. The study was organized as a four-week summer camp implementing a strict GF/DF diet with prospective collection of blood, urine and stool in addition to whole exome sequencing WES of DNA of participants. Using flow cytometry, proteomic assays and microbiome metagenomics, we show that GF/DF diet had a major anti-inflammatory effect in all participants both at the protein and cellular level with 4-fold increase in T regulatory/T helper 17 cells ratio and the promotion of a favorable regulatory gut microbiota. Overall, GF/DF can have a significant anti-inflammatory effect in children with SRNS and further trials are warranted to investigate this potential dietary intervention in children with SRNS.
KW - dairy-free
KW - diet
KW - gluten-free
KW - inflammation
KW - steroid resistance nephrotic syndrome
UR - http://www.scopus.com/inward/record.url?scp=85108097712&partnerID=8YFLogxK
U2 - 10.3389/fimmu.2021.624821
DO - 10.3389/fimmu.2021.624821
M3 - Article
C2 - 34149688
AN - SCOPUS:85108097712
SN - 1664-3224
VL - 12
JO - Frontiers in immunology
JF - Frontiers in immunology
M1 - 624821
ER -